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Clinical Chemistry 34: 382-384, 1988;
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Clinical Chemistry, Vol 34, 382-384, Copyright © 1988 by American Association for Clinical Chemistry

Improved assay for bismuth in biological samples by atomic absorption spectrophotometry with hydride generation

PR Froomes, AT Wan, PM Harrison and AJ McLean
Department of Clinical Pharmacology, Alfred Hospital, Prahran, Victoria, Australia.

This simple, rapid, sensitive, reliable, and economical assay for bismuth in plasma, erythrocytes, and urine is based on atomic absorption spectrophotometry with hydride generation. Acid digestion eliminates the problem of foaming, which hitherto has complicated such assay of bismuth in plasma and erythrocytes. The detection limit of the assay has been improved to 0.1 micrograms/L, as compared with a previously documented limit of 2.5 micrograms/L. Average recovery exceeded 95% in all biological fluids. Economy of use derives from elimination of need for electrodeless discharge lamps and atomic absorption grade borohydride. Determination of basal concentrations of bismuth in clinical samples of body fluids gave reference intervals of 0.1-3.5 micrograms/L for plasma, 0.3-4.6 micrograms/L for urine.


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[Abstract] [Full Text]


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