Clinical Chemistry AACC Online Job Center
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 34: 385-388, 1988;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Adachi, Y.
Right arrow Articles by Yamamoto, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Adachi, Y.
Right arrow Articles by Yamamoto, T.

Clinical Chemistry, Vol 34, 385-388, Copyright © 1988 by American Association for Clinical Chemistry

Clinical application of serum bilirubin fractionation by simplified liquid chromatography

Y Adachi, H Inufusa, M Yamashita, A Kambe, K Yamazaki, Y Sawada and T Yamamoto
Second Department of Internal Medicine, Kinki University School of Medicine, Osaka, Japan.

Serum bilirubin was fractionated by a new reversed-phase "high- performance" liquid-chromatographic (HPLC) procedure, on Micronex RP- 30, a polyacryl ester. The five fractions were: delta (delta-bilirubin, B delta), gamma (bilirubin diglucuronide, BDG), beta (bilirubin monoglucuronide, BMG), beta' [(Z,E)- and (or) (E,Z)-bilirubin IX alpha], and alpha [(Z,Z)-bilirubin IX alpha]. We found close correlation with results of the modified HPLC fractionation of Lauff et al. (J Chromatogr 1981;226:391-402), except for the beta' fraction, which was eluted after beta. The Micronex HPLC involves a simple pretreatment of serum samples, in contrast with the complex preparation described by Lauff et al., and is convenient for routine use in the clinical evaluation of hyperbilirubinemia. We could quantify B delta, BDG, BMG, and unconjugated bilirubin even in sera with normal values for total-bilirubin concentrations. Photoderivatives of bilirubin such as the beta' fraction could be separated and quantified by the same procedure, making the method feasible for pediatric research.


The following articles in journals at HighWire Press have cited this article:


Home page
 Clin. Chem.Home page
B. T. Doumas, F. Yein, B. Perry, B. Jendrzejczak, and A. Kessner
Determination of the Sum of Bilirubin Sugar Conjugates in Plasma by Bilirubin Oxidase
Clin. Chem., August 1, 1999; 45(8): 1255 - 1260.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1988 by the American Association for Clinical Chemistry.