Clinical Chemistry AACC Online Job Center
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 34: 528-530, 1988;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Imai, Y.
Right arrow Articles by Fujita, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Imai, Y.
Right arrow Articles by Fujita, K.

Clinical Chemistry, Vol 34, 528-530, Copyright © 1988 by American Association for Clinical Chemistry

Simultaneous determination of catecholamines and serotonin by liquid chromatography, after treatment with boric acid gel

Y Imai, S Ito, K Maruta and K Fujita
Institute for Comprehensive Medical Science, Fujita-Gakuen Health University, Aichi, Japan.

We describe a liquid-chromatographic method for simultaneously determining norepinephrine, epinephrine, dopamine, and serotonin in 0.5 mL of human plasma. These analytes are purified on boric acid gel from Aldrich, separated on a reversed-phase C18 column, and detected electrochemically at +600 mV. Absolute recoveries of internal standards were 84% for 3,4-dihydroxybenzylamine and 57% for N-methylserotonin. Reproducibility was good to excellent, depending on the concentration of the analytes. A chromatographic run is complete in 40 min, but this can be shortened by about half when the determination of only serotonin is required, by increasing the column temperature from 40 degrees C to 60 degrees C.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1988 by the American Association for Clinical Chemistry.