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Clinical Chemistry 34: 531-534, 1988;
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Clinical Chemistry, Vol 34, 531-534, Copyright © 1988 by American Association for Clinical Chemistry

Measurement of cholesterol in serum by gas chromatography/mass spectrometry at moderate mass resolution, with a nonendogenous cholesterol isomer as internal standard

M Kinter, DA Herold, J Hundley, MR Wills and J Savory
Department of Pathology, University of Virginia Medical Center, Charlottesville 22908.

We describe a gas chromatography/mass spectrometry method for the quantitative analysis of cholesterol in serum. A structural isomer of cholesterol, 7,(5 alpha)-cholesten-3 beta-ol, is used as an internal standard, its primary advantage being its lesser cost relative to that of a stable-isotope-labeled analog. Analysis of the National Bureau of Standards Certified Reference Serum (SRM 909) was used to validate the method. The results show this method to be highly accurate (bias = - 0.6%) and precise (CV = 1.6% between-run, 1.2% within-run). The performance of this method is, therefore, sufficiently good to allow its use as a reference method for determinations of cholesterol in serum.


The following articles in journals at HighWire Press have cited this article:


Home page
 Clin. Chem.Home page
R. Kock, B. Delvoux, and H. Greiling
Determination of total cholesterol in serum by liquid chromatography–isotope dilution mass spectrometry
Clin. Chem., October 1, 1997; 43(10): 1896 - 1903.
[Abstract] [Full Text] [PDF]


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Copyright © 1988 by the American Association for Clinical Chemistry.