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Clinical Chemistry 34: 661-664, 1988;
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Clinical Chemistry, Vol 34, 661-664, Copyright © 1988 by American Association for Clinical Chemistry

Measurement of ferritin in serum by an indirect competitive enzyme- linked immunosorbant assay

RJ Polson, JG Kenna, IP Shears, A Bomford and R Williams
Liver Unit, King's College Hospital, London, U.K.

This indirect competitive enzyme-linked immunosorbant assay (ELISA) for ferritin, unlike other currently available ELISAS, does not require use of an anti-ferritin antibody-enzyme conjugate. Designed for use on microtiter plates, the method has a precision and sensitivity similar to those of other immunoassays. The detection limit is 20 pg of ferritin per test (corresponding to 2.0 micrograms/L in serum samples). Comparison of results obtained on serum from 57 patients by this method with those from a conventional radioimmunoassay gave a correlation coefficient of 0.92.


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Copyright © 1988 by the American Association for Clinical Chemistry.