Clinical Chemistry Link to Randox Laboratories Web Site
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 34: 676-679, 1988;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ha, H. R.
Right arrow Articles by Thomson, D. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ha, H. R.
Right arrow Articles by Thomson, D. A.

Clinical Chemistry, Vol 34, 676-679, Copyright © 1988 by American Association for Clinical Chemistry

Midazolam in plasma from hospitalized patients as measured by gas- liquid chromatography with electron-capture detection

HR Ha, B Funk, PO Maitre, AM Zbinden, F Follath and DA Thomson
Department of Anesthesia, University of Basle/Kantonsspital, Switzerland.

The present assay was developed for quantifying midazolam in plasma of patients hospitalized in intensive-care units or undergoing anesthesia and receiving many other drugs as well. Plasma samples are alkalinized with NaOH and midazolam is extracted into n-hexane. The organic phase is evaporated and reconstituted in n-butyl acetate, and the midazolam is quantified by gas-liquid chromatography with electron-capture detection. The calibration graph for midazolam was linear in the ranges 5-200 and 200-800 micrograms/L. The CVs for precision and reproducibility of the assay were less than 8%. The method was very specific for midazolam; most of the drugs commonly used in anesthesia and in the intensive-care unit did not interfere with the assay. The lowest detectable concentration was 1 microgram/L. The method is adaptable for use with an automated chromatographic system.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1988 by the American Association for Clinical Chemistry.