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Clinical Chemistry 34: 954-957, 1988;
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Clinical Chemistry, Vol 34, 954-957, Copyright © 1988 by American Association for Clinical Chemistry

Urinary alanine aminopeptidase assay improved as result of multivariate response-surface analysis

C Flandrois, C Lahet, D Feldmann, JM Gabastou, A Gonnon and I Maire
Laboratoire d'Enzymologie (Monique Mathieu), Hopital Debrousse, Lyon, France.

Optimization of determination of alanine aminopeptidase in urine by univariate study led to a method involving pretreatment of urine with Sephadex G50. Re-examination of the optimization by multivariate study led us to recommend higher optimal concentrations: 5.8 mmol/L for the substrate and 300 mmol/L for the Tris buffer. Under these new conditions, pretreatment of urine was no longer necessary and the assay could be completely automated.




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