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Clinical Chemistry, Vol 35, 1371-1375, Copyright © 1989 by American Association for Clinical Chemistry
RA Magnotti Jr, GW Stephens, RK Rogers and AJ Pesce
Department of Internal Medicine, University of Cincinnati, OH 45267- 0585.
We describe microplate methods for measurement of human urinary albumin (HUA) by competitive enzyme-linked immunosorbant assay (ELISA) and creatinine with a modified commercial enzymatic kit. Incorporation of substrate mixing into the competitive ELISA changes the dynamic absorbance-concentration response, greatly simplifying calculations and improving sensitivity and accuracy. Measurement of creatinine in urine and plasma samples with a commercially available enzymatic kit modified for analysis by use of an inexpensive microplate reader produced values comparable in precision and accuracy to those obtained by an automated kinetic Jaffe method.
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