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Clinical Chemistry 35: 1371-1375, 1989;
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Clinical Chemistry, Vol 35, 1371-1375, Copyright © 1989 by American Association for Clinical Chemistry

Microplate measurement of urinary albumin and creatinine

RA Magnotti Jr, GW Stephens, RK Rogers and AJ Pesce
Department of Internal Medicine, University of Cincinnati, OH 45267- 0585.

We describe microplate methods for measurement of human urinary albumin (HUA) by competitive enzyme-linked immunosorbant assay (ELISA) and creatinine with a modified commercial enzymatic kit. Incorporation of substrate mixing into the competitive ELISA changes the dynamic absorbance-concentration response, greatly simplifying calculations and improving sensitivity and accuracy. Measurement of creatinine in urine and plasma samples with a commercially available enzymatic kit modified for analysis by use of an inexpensive microplate reader produced values comparable in precision and accuracy to those obtained by an automated kinetic Jaffe method.


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Copyright © 1989 by the American Association for Clinical Chemistry.