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Clinical Chemistry 35: 1408-1415, 1989;
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Clinical Chemistry, Vol 35, 1408-1415, Copyright © 1989 by American Association for Clinical Chemistry

Radioimmunoassay of osteocalcin with polyclonal and monoclonal antibodies

MJ Power, JP Gosling and PF Fottrell
Department of Biochemistry, University College, Galway, Ireland.

We have developed and thoroughly validated radioimmunoassays (RIAs) for osteocalcin in plasma and serum, demonstrating independence of volume and determining the recovery of added standard and within- and between- assay precision. Antibodies were raised in rabbits and mice by immunization with either osteocalcin adsorbed to polyvinylpyrrolidone or osteocalcin conjugated to bovine serum albumin. In both species, use of the conjugated osteocalcin was more efficacious. Correlation of the results obtained when groups of plasma or serum samples were analyzed by RIAs with different antibodies (two polyclonal and one monoclonal) indicated that the normal range observed for osteocalcin concentrations in serum depends on the antibody used. These findings may account for the wide range of "normal" osteocalcin values reported by different groups.


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Vitamin K Status and Bone Health: An Analysis of Methods for Determination of Undercarboxylated Osteocalcin
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Copyright © 1989 by the American Association for Clinical Chemistry.