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Clinical Chemistry 35: 1945-1948, 1989;
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Clinical Chemistry, Vol 35, 1945-1948, Copyright © 1989 by American Association for Clinical Chemistry

HPLC analysis of brain and plasma for octanoic and decanoic acids

HG Dean, JC Bonser and JP Gent
Department of Pharmacology, University of Leeds, U.K.

Two methods are described for determination of octanoic and decanoic acids in plasma and brain homogenate by "high-performance" liquid chromatography with ultraviolet detection. Analysis of the underivatized acids had a detection limit of only 50 mg/L, but formation of the p-bromophenacyl ester increased the sensitivity by 100- fold, to a detection limit of 0.5 mg/L. The latter procedure gave interassay coefficients of variation of 4.1% and 4.8% for octanoic and decanoic acids, respectively. The corresponding intra-assay values were 3.95% and 4.7% (n = 6). The derivative method, applied to samples of plasma from children receiving a medium-chain triglyceride (MCT) diet, gave values in agreement with results by gas-liquid chromatography. Results have also been obtained for samples from mice, either treated with the medium-chain triglyceride diet or given infusions of sodium octanoate.




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Copyright © 1989 by the American Association for Clinical Chemistry.