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Clinical Chemistry 37: 1645-1648, 1991;
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Clinical Chemistry, Vol 37, 1645-1648, Copyright © 1991 by American Association for Clinical Chemistry

Development of an enzyme-linked immunosorbent assay of apolipoprotein E- AII complex in plasma

M Tozuka, Y Yoshida, J Tanigami, M Miyachi, T Katsuyama and M Kanai
Central Clinical Laboratories, Shinshu University Hospital, Matsumoto, Japan.

Measurement of apolipoprotein (apo) E-AII complex in human plasma is important in determining the role of apoE in lipoprotein metabolism. In this paper, we demonstrate a new and simple method to determine apoE- All complex by using an enzyme-linked immunosorbent assay. Anti-apoE IgG (goat) was used as a capture antibody, and captured apoE-All complexes were detected by an anti-apoAll (rabbit) horseradish peroxidase-conjugated anti-rabbit IgG (goat) system. With this method, apoE-All complex was specifically determined without the interference of apoAll and was not affected by apoE monomer less than 250 mg/L. The content of the complex in reference serum, a normolipidemic serum pooled from five subjects with phenotype E3/E3, was arbitrarily defined as 100%. The coefficients of variation were 3.5%-6.3% within assay and 8.8%-11.6% between assays.




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Copyright © 1991 by the American Association for Clinical Chemistry.