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Clinical Chemistry 38: 123-126, 1992;
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Clinical Chemistry, Vol 38, 123-126, Copyright © 1992 by American Association for Clinical Chemistry

Monoclonal fluorescence polarization immunoassay evaluated for monitoring cyclosporine in whole blood after kidney, heart, and liver transplantation

M Winkler, G Schumann, D Petersen, M Oellerich and K Wonigeit
Klinik fur Abdominal und Transplantationschirurgie, Medizinische Hochschule Hannover, F.R.G.

In a prospective study we evaluated a novel fluorescence polarization immunoassay (FPIA) for determining cyclosporine (CsA) in whole blood. FPIA uses a monoclonal antibody and is performed on the TDx (Abbott). The within-series (CV less than 2%) and between-days (CV less than 3.3%) precision of the assay was excellent. The results obtained by the monoclonal FPIA in samples from transplant patients (n = 100) averaged 31.9% and 20.2% higher than those by HPLC and a specific radioimmunoassay (INCStar), respectively. Results by all three methods correlated well. Follow-up studies during the early course after liver transplantation, however, suggested that high metabolite concentrations affect FPIA results. This is explained by previously described cross- reactions of the monoclonal antibody with some CsA metabolites. The FPIA results in samples of such patients should be interpreted cautiously.


The following articles in journals at HighWire Press have cited this article:


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 Clin. Chem.Home page
W. Steimer
Performance and Specificity of Monoclonal Immunoassays for Cyclosporine Monitoring: How Specific Is Specific?
Clin. Chem., March 1, 1999; 45(3): 371 - 381.
[Abstract] [Full Text] [PDF]

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L. J. Aspeslet, D. F. LeGatt, G. Murphy, and R. W. Yatscoff
Effect of assay methodology on pharmacokinetic differences between cyclosporine Neoral(R) and Sandimmune(R) formulations
Clin. Chem., January 1, 1997; 43(1): 104 - 108.
[Abstract] [Full Text] [PDF]


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Copyright © 1992 by the American Association for Clinical Chemistry.