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Clinical Chemistry 38: 144-147, 1992;
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Clinical Chemistry, Vol 38, 144-147, Copyright © 1992 by American Association for Clinical Chemistry

Simple quantitative measurement of serum choriogonadotropin compared with immunoradiometric, immunoenzymometric, and chemiluminescent assays

RJ Norman, TA Gilmore and JW McLoughlin
University of Adelaide, Department of Obstetrics and Gynaecology, Queen Elizabeth Hospital, Woodville, South Australia.

We evaluated a new simplified quantitative method (Tandem Icon QSR; Hybritech) for choriogonadotropin (hCG), which could theoretically be performed in a ward or with outpatients. The method was compared with immunoradiometric (Bioclone Australia), chemiluminescent (Amerlite; Amersham), and immunoenzymometric assays (Stratus; Dade). We analyzed by each of the methods 104 serum samples from pregnant and nonpregnant patients. For quantitative hCG values indicative of pregnancy (greater than 25 int. units/L), excellent correlation was observed between the Tandem Icon method and the other standard laboratory assays (r = 0.995, 0.990, and 0.992, respectively). Occasional problems arose because of the instability of Tandem Icon QSR reagents at room temperature but this was resolved by storing the reagents at 4 degrees C. We conclude that this simplified quantitative method for hCG is reliable and suitable for use outside of the routine immunoassay laboratory.




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Copyright © 1992 by the American Association for Clinical Chemistry.