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Clinical Chemistry 38: 2061-2065, 1992;
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Clinical Chemistry, Vol 38, 2061-2065, Copyright © 1992 by American Association for Clinical Chemistry

Flow-injection analysis for malondialdehyde in plasma with the thiobarbituric acid reaction

H Ikatsu, T Nakajima, N Murayama and T Korenaga
Department of Hygiene, Shinshu University School of Medicine, Matsumoto- shi, Japan.

A simple, precise, and rapid method to measure plasma malondialdehyde (MDA) was developed by use of solvent extraction--flow-injection analysis. The reagent solution, containing thiobarbituric acid (TBA), 5 g/L in 100 mL/L phosphoric acid, and extraction solvent (methylisobutyl ketone, MIBK) were propelled with a double-plunger micropump at a flow rate of 0.3 mL/min, and 20 microL of sample was introduced into the reagent stream. After TBA-MDA reactant was extracted into MIBK, the organic phase was continuously separated by a successive phase- separation system equipped with two phase separators, and the absorbance of the TBA-MDA reactant was measured at 532 nm. This approach resulted in excellent sensitivity, a CV of < 1.5%, a good correlation with the conventional manual method, and a sampling frequency of 7 samples/h, suggesting that this semiautomated method is suitable for measuring plasma MDA.




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Copyright © 1992 by the American Association for Clinical Chemistry.