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Clinical Chemistry 38: 2267-2272, 1992;
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Clinical Chemistry, Vol 38, 2267-2272, Copyright © 1992 by American Association for Clinical Chemistry

Immunoluminometric assay of chromogranin A in serum with commercially available reagents

H Bender, A Maier, B Wiedenmann, DT O'Connor, K Messner and H Schmidt-Gayk
Endocrine Laboratory, Heidelberg, FRG.

Chromogranin A (Cg A) is a useful marker of neuroendocrine neoplasia in humans. Here we describe an immunoluminometric assay (ILMA) for measuring Cg A in serum, with use of a new tube as a solid phase. The new tube has a large surface area and is coated with a polyclonal antibody. Optimized coating conditions provide a high IgG adsorption to the polystyrene wall. Serum is added to the coated tube and incubated for 2 h or overnight. After washing the tubes, acridinium ester-labeled monoclonal antibody against Cg A is added and incubation is continued for 2 h. The tubes are washed again and the bound luminescence is measured. The assay is very sensitive (detection limit 1 microgram/L, if 25 microL of serum is used), is specific for human Cg A, and offers a wide dynamic range (1-1000 micrograms/L). The range in healthy humans is 10-53 micrograms/L (median 30 micrograms/L). The correlation of serum Cg A to creatinine and parathyroid hormone is described. We also report the influence of intravenous calcium injection on Cg A concentrations in serum.


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Copyright © 1992 by the American Association for Clinical Chemistry.