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Clinical Chemistry 38: 766-768, 1992;
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Clinical Chemistry, Vol 38, 766-768, Copyright © 1992 by American Association for Clinical Chemistry

Nonisotopic binding assay for measuring vitamin B12 and folate in serum

J van der Weide, HC Homan, E Cozijnsen-van Rheenen, Y Vivie-Kipp, J Poortman and RJ Kraaijenhagen
Department of Clinical Chemistry and Hematology, Hospital De Lichtenberg, Amersfoort, The Netherlands.

We evaluated a nonisotopic method (CEDIA; cloned enzyme donor immunoassay) for estimating vitamin B12 and folate in serum. The assays were performed with a Cobas-Mira analyzer. Intra-assay CVs were from 3.7% to 11.0% for vitamin B12 and from 1.2% to 10.7% for folate. Interassay CVs ranged from 9.5% to 11.9% for vitamin B12 and from 6.1% to 18.5% for folate. Linearity was satisfactory, with analytical recovery of 94% at 8.7 and 25.4 nmol/L for folate and 280 and 554 pmol/L for vitamin B12. The detection limit was 3.6 nmol/L for folate and 12.3 pmol/L for vitamin B12. Results of this assay correlated well with those of a conventional radioassay: r = 0.98 for vitamin B12 (n = 51) and r = 0.97 for folate (n = 57). The CEDIA was easy to perform but appeared to be unreliable for use with samples from myeloma patients.




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Copyright © 1992 by the American Association for Clinical Chemistry.