Clinical Chemistry Link to Randox Laboratories Web Site
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 4: 409-419, 1958;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Balikov, B.
Right arrow Articles by Castello, R. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Balikov, B.
Right arrow Articles by Castello, R. A.

Clinical Chemistry, Vol 4, 409-419, Copyright © 1958 by the American Association for Clinical Chemistry

The Assay of Urinary Peptides Using a Biuret Reagent

Bernard Balikov 1, Eloy R. Lozano 1, and Robert A. Castello 1

1 Department of Metabolism, Division of Medicine, WRAIR, Walter Reed Army Medical Center, Washington, D. C.

The components of a biuret reagent have been investigated to minimize variations in readings resulting from small variations in composition. When this biuret reagent is used for the assay of urinary peptides, readings should be made at 405 mµ. There is a limited but adequate straight-line relationship between concentration and absorbance on normal urine. Urine from convalescing burn patients containing normal concentrations of peptides may read 10-20 per cent low.

This method has a coefficient of variation of 1.68 per cent. Under ordinary circumstances there will be no interference from oxalic acid, creatinine, uric acid, ammonia, or hippuric acid. Amino acids and glucose may result in false elevations of readings. Samples must be preserved by refrigeration or with toluene or thymol, and when so treated are stable up to 48 hours for peptide assay.

Submitted on April 7, 1958






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1958 by the American Association for Clinical Chemistry.