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Clinical Chemistry 40: 2030-2034, 1994;
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Clinical Chemistry, Vol 40, 2030-2034, Copyright © 1994 by American Association for Clinical Chemistry

Assay of plasma oxalate with soluble oxalate oxidase

M Petrarulo, E Cerelli, M Marangella, D Cosseddu, C Vitale and F Linari
Renal Stone Laboratory, Mauriziano Umberto I Hospital, Turin, Italy.

We use oxalate oxidase from barley seedlings for the colorimetric determination of oxalate in plasma. The oxalate is converted to hydrogen peroxide, which, in the presence of peroxidase, is detected by a Trinder-like chromogenic system. Optimization of the assay, including deproteinization and elimination of interferences from reducing substrates, is described. Ascorbate additions (200 mumol/L) did not affect oxalate concentration in plasma, even after long frozen storage. Mean analytical recovery of oxalate averaged 102% +/- 6.9%, imprecision (CV) at 2.0 mumol/L was 7.2%, and the lower limit of quantification (CV = 20%) was 0.6 mumol/L. Results correlated well with those by chromatography (r = 0.999, Sy/x = 0.29 mumol/L, n = 32, range for x, y = 0-140 mumol/L). Plasma oxalate concentrations measured in 32 healthy subjects ranged from 0.6 to 2.9 mumol/L (mean 1.28, SD 0.71 mumol/L), which agrees with those measurable by using indirect radioisotopic dilution methods. Patients with primary hyperoxaluria and chronic renal failure exhibited markedly greater plasma concentrations of oxalate.


The following articles in journals at HighWire Press have cited this article:


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 Clin. Chem.Home page
B. G. Keevil and S. Thornton
Quantification of Urinary Oxalate by Liquid Chromatography-Tandem Mass Spectrometry with Online Weak Anion Exchange Chromatography
Clin. Chem., December 1, 2006; 52(12): 2296 - 2299.
[Abstract] [Full Text] [PDF]

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 Clin. Chem.Home page
P. M. Ladwig, R. R. Liedtke, T. S. Larson, and J. C. Lieske
Sensitive Spectrophotometric Assay for Plasma Oxalate
Clin. Chem., December 1, 2005; 51(12): 2377 - 2380.
[Full Text] [PDF]


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