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Clinical Chemistry 43: 533-538, 1997;
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(Clinical Chemistry. 1997;43:533-538.)
© 1997 American Association for Clinical Chemistry, Inc.

Articles

Enzymatic assay of D-mannose in serum

James R. Etchison and Hudson H. Freezea

The Burnham Institute, Glycobiology/Carbohydrate Chemistry Program, 10901 North Torrey Pines Rd., La Jolla, CA 92037
a Author for correspondence: Fax 619-646-3193; email hudson{at}ljcrf.edu

We describe a new and improved enzymatic assay for determining the concentration of D-mannose in sera. Serum D-glucose is selectively converted to glucose-6 phosphate with the highly specific thermostable glucokinase (EC 2.7.1.2) from Bacillus stearothermophilus. The anionic reaction products and excess substrates are removed by a rapid and simple anion-exchange chromatography step in microcentrifuge spin columns. D-Mannose in the glucose-depleted sample is then assayed spectrophotometrically by using coupled enzymatic reactions. The quantitative elimination of glucose from the serum samples allowed the accurate and reproducible assay of serum mannose in the 0–200 µmol/L range. Recovery of mannose added to serum (5–200 µmol/L) was 94% ± 4.4%. The intraassay CV was 6.7% at 40 µmol/L mannose (n = 5; 39.6 ± 1.6 µmol/L) and 4.4% at 80 µmol/L (n = 11; 75.0 ± 1.8 µmol/L); the interassay CV at these concentrations was 12.2% (n = 7; 36.9 ± 2.1 µmol/L) and 9.8% (n = 7; 74.2 ± 2.7 µmol/L), respectively. Sera from 11 healthy human volunteers contained an average of 54.1 ± 11.9 µmol/L mannose (range 36–81 µmol/L).


Key Words: indexing terms: glucose oxidase • glucokinase • carbohydrate-deficient glycoprotein syndrome • phosphomannose isomerase • phosphoglucose isomerase




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