Clinical Chemistry
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Clinical Chemistry 43: 1029-1032, 1997;
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(Clinical Chemistry. 1997;43:1029-1032.)
© 1997 American Association for Clinical Chemistry, Inc.

Articles

Simultaneous identification and quantitation of codeine, morphine, hydrocodone, and hydromorphone in urine as trimethylsilyl and oxime derivatives by gas chromatography–mass spectrometry

Larry A. Broussard1,a, Lance C. Presley2, Thomas Pittman3, Randy Clouette4 and Gary H. Wimbish4

1 Department of Medical Technology, LSU Medical Center, New Orleans, LA 70112-2262.

2 LabCorp, Memphis, TN 38118.

3 TF Puckett Laboratory, Hattiesburg, MS 39402.

4 Harrison Laboratories, Midland, TX 79706.
a Author for correspondence. Fax 504-568-6761; e-mail lbrous{at}lsumc.edu

Following enzymatic hydrolysis of urine, a gas chromatography–mass spectrometry method for the simultaneous determination of codeine, morphine, hydrocodone, and hydromorphone uses hydroxylamine to form oxime derivatives of the keto-opiates (i.e., hydrocodone, hydromorphone, oxycodone, and oxymorphone). These trimethylsilyl-derivatized forms no longer interfere with the detection and quantitation of codeine and morphine. Samples are extracted on solid-phase columns and quantitated by deuterated internal calibrations of each analyte with selected ion monitoring. Codeine, morphine, hydrocodone, and hydromorphone are completely separated, allowing simultaneous quantitation without interference and a chromatographic analysis time <9 min.


Key Words: indexing terms: abused drugs • drug monitoring • opiates • forensic toxicology




The following articles in journals at HighWire Press have cited this article:


Home page
 Clin. Chem.Home page
L. A. Broussard, L. C. Presley, M. Tanous, and C. Queen
Improved Gas Chromatography-Mass Spectrometry Method for Simultaneous Identification and Quantification of Opiates in Urine as Propionyl and Oxime Derivatives
Clin. Chem., January 1, 2001; 47(1): 127 - 129.
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