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Clinical Chemistry 43: 1582-1587, 1997;
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(Clinical Chemistry. 1997;43:1582-1587.)
© 1997 American Association for Clinical Chemistry, Inc.

Articles

International collaborative study to evaluate a recombinant L ferritin preparation as an International Standard

Susan J. Thorpea, Dawn Walker, Paolo Arosio1, Alan Heath, James D. Cook2 and Mark Worwood3

1 University of Brescia, Brescia, Italy.

2 University of Kansas Medical Center, Kansas City, KS, USA.

3 University of Wales College of Medicine, Cardiff, UK.
a Author for correspondence: Division of Hematology, NIBSC, Blanche Lane, South Mimms, Potters Bar, Herts EN6 3QG, UK. Fax 44-(0)1707-646730; e-mail sthorpe{at}nibsc.ac.uk

A recombinant L ferritin preparation, lyophilized in ampoules and designated 94/572, was evaluated by 18 laboratories in 9 countries for its suitability as an International Standard (IS). The preparation was assayed in a wide range of in-house and commercial immunoassays against the 2nd IS for ferritin (of spleen origin; 80/578). The immunological reactivity of the recombinant material was similar to that of the 2nd IS for ferritin in the majority of assays and demonstrated adequate stability in accelerated degradation studies. On the basis of the results presented here, the WHO Expert Committee on Biological Standardization established 94/572 as the 3rd IS for ferritin, recombinant.






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Copyright © 1997 by the American Association for Clinical Chemistry.