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Clinical Chemistry 45: 262-268, 1999;
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(Clinical Chemistry. 1999;45:262-268.)
© 1999 American Association for Clinical Chemistry, Inc.

Articles

Coupled-Column Liquid Chromatographic Analysis of Catecholamines, Serotonin, and Metabolites in Human Urine

Torsten J. Panholzer, Jürgen Beyer and Klaus Lichtwalda

a Author for correspondence. Fax 49-6131-176619; e-mail lichtwal{at}mail.uni-mainz.de.

Acolumn-switching HPLC system was utilized for the simultaneous determination of epinephrine, norepinephrine, dopamine, serotonin, and their metabolites metanephrine, normetanephrine, 3,4-dihydroxyphenylacetic acid, homovanillic acid, and 5-hydroxyindoleacetic acid in human urine. The sample was injected directly onto a C18-alkyl-diol silica precolumn, which separated the analytes from matrix. The analytes were eluted from the precolumn onto the analytical column by the use of column-switching techniques and were then separated on the analytical column by means of ion-pair reversed-phase HPLC. The analytes were then oxidized to the corresponding quinones and converted into fluorescent derivatives by reaction with meso-1,2-diphenylethylenediamine.




The following articles in journals at HighWire Press have cited this article:


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 Clin. Chem.Home page
R. L. Taylor and R. J. Singh
Validation of Liquid Chromatography-Tandem Mass Spectrometry Method for Analysis of Urinary Conjugated Metanephrine and Normetanephrine for Screening of Pheochromocytoma
Clin. Chem., March 1, 2002; 48(3): 533 - 539.
[Abstract] [Full Text] [PDF]

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 Clin. Chem.Home page
I. P. Kema, W. G. Meijer, G. Meiborg, B. Ooms, P. H.B. Willemse, and E. G.E. de Vries
Profiling of Tryptophan-related Plasma Indoles in Patients with Carcinoid Tumors by Automated, On-Line, Solid-Phase Extraction and HPLC with Fluorescence Detection
Clin. Chem., October 1, 2001; 47(10): 1811 - 1820.
[Abstract] [Full Text] [PDF]


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