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Clinical Chemistry 46: 529-536, 2000;
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(Clinical Chemistry. 2000;46:529-536.)
© 2000 American Association for Clinical Chemistry, Inc.

Articles

Simple Microplate Method for Determination of Urinary Iodine

Toshinori Ohashi1,a, Mitsuo Yamaki1, Chandrakant S. Pandav2, Madhu G. Karmarkar2 and Minoru Irie3

1 Pharmaceutical Research Laboratory, Hitachi Chemical Co., Ltd., 13-1, Higashi-cho 4-chome, Hitachi-shi, Ibaraki-ken 317-8555, Japan.

2 International Council for Control of Iodine Deficiency Disorders, New Delhi 110029, India.

3 International Council for Control of Iodine Deficiency Disorders, Tokyo 112-0001, Japan.
a Author for correspondence. Fax 81-294-24-3602; e-mail tos-oohashi{at}hitachi-chem.co.jp

Background: Urinary iodine is a good biochemical marker for control of iodine deficiency disorders. Our aim was to develop and validate a simple, rapid, and quantitative method based on the Sandell–Kolthoff reaction, incorporating both the reaction and the digestion process into a microplate format.

Methods: Using a specially designed sealing cassette to prevent loss of vapor and cross-contamination among wells, ammonium persulfate digestion was performed in a microplate in an oven at 110 °C for 60 min. After the digestion mixture was transferred to a transparent microplate and the Sandell–Kolthoff reaction was performed at 25 °C for 30 min, urinary iodine was measured by a microplate reader at 405 nm.

Results: The mean recovery of iodine added to urine was 98% (range, 89–109%). The theoretical detection limit, defined as 2 SD from the zero calibrator, was 0.11 µmol/L (14 µg/L iodine). The mean intra- and interassay CVs for samples with iodine concentrations of 0.30–3.15 µmol/L were <=10%. The new method agreed well with the conventional chloric acid digestion method (n = 70; r = 0.991; y = 0.944x + 0.04; Sy|x = 0.10) and with the inductively coupled plasma mass spectrometry method (n = 61; r = 0.979; y = 0.962x + 0.03; Sy|x = 0.20). The agreement was confirmed by difference plots. The distributions of iodine concentrations for samples from endemic areas of iodine deficiency diseases showed similar patterns among the above three methods.

Conclusions: Our new method, incorporating the whole process into a microplate format, is readily applicable and allows rapid monitoring of urinary iodine.




The following articles in journals at HighWire Press have cited this article:


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S. Fallouch, P.-J. Lejeune, J. Barbaria, P. Carayon, and B. Mallet
Urinary Iodine Analysis: An Alternative Method for Digestion of Urine Samples
Clin. Chem., April 1, 2004; 50(4): 780 - 782.
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M. B Zimmermann, S. Y Hess, L. Molinari, B. de Benoist, F. Delange, L. E Braverman, K. Fujieda, Y. Ito, P. L Jooste, K. Moosa, et al.
New reference values for thyroid volume by ultrasound in iodine-sufficient schoolchildren: a World Health Organization/Nutrition for Health and Development Iodine Deficiency Study Group Report
Am. J. Clinical Nutrition, February 1, 2004; 79(2): 231 - 237.
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D. Gnat, A. D. Dunn, S. Chaker, F. Delange, F. Vertongen, and J. T. Dunn
Fast Colorimetric Method for Measuring Urinary Iodine
Clin. Chem., January 1, 2003; 49(1): 186 - 188.
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 Asia Pac J Public HealthHome page
C. Yamada, D. Oyunchimeg, P. Enkhtuya, A. Erdenbat, A. Buttumur, and T. Umenai
Current Status of Iodine Deficiency in Mongolia in 1998-1999
Asia Pac J Public Health, January 1, 2000; 12(2): 79 - 84.
[Abstract] [PDF]


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