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Clinical Chemistry 46: 901-906, 2000;
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(Clinical Chemistry. 2000;46:901-906.)
© 2000 American Association for Clinical Chemistry, Inc.

Articles

Quantitative Analysis of Aldolase A mRNA in Liver Discriminates between Hepatocellular Carcinoma and Cirrhosis

Giuseppe Castaldo1,4, Giuseppe Calcagno1, Raffaella Sibillo1, Rosario Cuomo2, Gerardo Nardone2, Luigi Castellano3, Camillo Del Vecchio Blanco3, Gabriele Budillon2 and Francesco Salvatore1,a

1 Dipartimento di Biochimica e Biotecnologie Mediche and CEINGE scarl,
2 Cattedra di Gastroenterologia, Facoltà di Medicina e Chirurgia, Università di Napoli "Federico II", I-80131 Naples, Italy.

3 Cattedra di Gastroenterologia, Seconda Università di Napoli, I-80131 Naples, Italy.

4 Facoltà di Scienze Matematiche, Fisiche e Naturali, Università del Molise, I-86170 Isernia, Italy.
a Address correspondence to this author at: Dipartimento di Biochimica e Biotecnologie Mediche, via S. Pansini 5, I-80131 Naples, Italy. Fax 39-081-7463650; e-mail salvator{at}unina.it

Background: Chronic liver diseases can progress to cirrhosis and to hepatocellular carcinoma. Timely and unequivocal recognition of the neoplastic evolution of cirrhosis is critical. To this aim, we used a noncompetitive reverse transcription-PCR procedure to analyze aldolase A mRNA in liver tissue from patients with chronic liver diseases at different stages.

Methods: We studied 12 patients with hepatocellular carcinoma, 19 patients affected by chronic hepatitis C or cirrhosis, and 7 healthy controls. Aldolase A mRNA was reverse-transcribed to cDNA, which was then amplified by PCR. The amplified segments were "read" with a novel dot-blot procedure. A calibrator with the same sequence, synthesized in vitro using a T7 phage promoter, was processed at scalar dilutions in parallel to the target samples to generate a calibration curve and so quantify the target mRNA (detection limit, 0.03 amol; linearity spanning five orders of magnitude).

Results: Aldolase A mRNA was ~10-fold higher in liver biopsies from patients with hepatocellular carcinoma vs patients with chronic hepatitis C or cirrhosis, and healthy individuals. Furthermore, aldolase A mRNA concentrations were 1.2- to 21.3-fold higher in 12 liver biopsies compared with the paired surrounding cirrhotic tissue.

Conclusions: The quantitative analysis of liver tissue aldolase A mRNA differentiates between nonneoplastic chronic liver diseases and hepatocellular carcinoma, which suggests that it has diagnostic potential.




The following articles in journals at HighWire Press have cited this article:


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 Eur Respir JHome page
G. Castaldo, R. Tomaiuolo, A. Sanduzzi, A. Ponticiello, I. Marchetiello, and F. Salvatore
Carcinoembryonic antigen mRNA analysis detects micrometastatic cells in blood from lung cancer patients
Eur. Respir. J., September 1, 2003; 22(3): 418 - 421.
[Abstract] [Full Text] [PDF]


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