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Clinical Chemistry 46: 1221-1229, 2000;
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(Clinical Chemistry. 2000;46:1221-1229.)
© 2000 American Association for Clinical Chemistry, Inc.

Articles

Flow Cytometry: Principles and Clinical Applications in Hematology

Michael Brown1 and Carl Wittwera,1

1 Department of Pathology, University of Utah, ARUP Laboratories, Inc., Salt Lake City, UT 84132.
a Address correspondence to this author at: Department of Pathology, University of Utah, 50 North Medical Dr., Salt Lake City, UT 84132. E-mail carl_wittwer{at}hlthsci.med.utah.edu

The use of flow cytometry in the clinical laboratory has grown substantially in the past decade. This is attributable in part to the development of smaller, user-friendly, less-expensive instruments and a continuous increase in the number of clinical applications. Flow cytometry measures multiple characteristics of individual particles flowing in single file in a stream of fluid. Light scattering at different angles can distinguish differences in size and internal complexity, whereas light emitted from fluorescently labeled antibodies can identify a wide array of cell surface and cytoplasmic antigens. This approach makes flow cytometry a powerful tool for detailed analysis of complex populations in a short period of time. This report reviews the general principles in flow cytometry and selected applications of flow cytometry in the clinical hematology laboratory.




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