HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit an electronic Letter to the Editor about this paper Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (4) Citing Articles via Google Scholar Google Scholar Articles by Piironen, T. Articles by Pettersson, K. Search for Related Content PubMed PubMed Citation Articles by Piironen, T. Articles by Pettersson, K. Related Collections Proteomics and Protein Markers

Measurement of Circulating Forms of Prostate-specific Antigen in Whole Blood Immediately after Venipuncture: Implications for Point-of-Care Testing

¹ The Finsen Laboratory af.sn. 8621, Strandboulevarden 49, 2100 Copenhagen, Denmark.

² Department of Biotechnology and
³ Paavo Nurmi Center, University of Turku, 20520 Turku, Finland.

⁴ Department of Surgery, Turku University Central Hospital, 20520 Turku, Finland.

⁵ Department of Clinical Chemistry, Turku University Central Hospital, 20520 Turku, Finland.

⁶ Department of Laboratory Medicine, Division of Clinical Chemistry, Lund University, University Hospital, 20502 Malmö, Sweden.
^a Author for correspondence. Fax 358-2-3338050; e-mail kim.pettersson{at}utu.fi.

Background: The purpose of this study was to validate the use of whole-blood samples in the determination of circulating forms of prostate-specific antigen (PSA).

Methods: Blood samples of hospitalized prostate cancer and benign prostatic hyperplasia patients were collected and processed to generate whole-blood and serum samples. Three different rapid two-site immunoassays were developed to measure the concentrations of total PSA (PSA-T), free PSA (PSA-F), and PSA-₁-antichymotrypsin complex (PSA-ACT) to detect in vitro changes in whole-blood samples immediately after venipuncture. The possible influence of muscle movement on the release of PSA from prostate gland was studied in healthy men by measuring the rapid in vitro whole-blood kinetics of PSA forms before and after 15 min of physical exercise on a stationary bicycle.

Results: Rapid PSA-T, PSA-F, and PSA-ACT assays were designed using a 10-min sample incubation. No significant changes were detected in the concentrations of PSA-T, PSA-F, and PSA-ACT from the earliest time point of 12–16 min compared with measurements performed up to 4 h after venipuncture. Physical exercise did not influence the concentrations of the circulating forms of PSA. Hematocrit-corrected whole-blood values of PSA-T and PSA-F forms were comparable to the respective serum values. Calculation of the percentage of PSA-F (PSA F/T ratio x 100) was similar irrespective of the sample format used, i.e., whole blood or serum.

Conclusions: We found that immunodetectable PSA forms are likely at steady state immediately after venipuncture, thus enabling the use of anticoagulated whole-blood samples in near-patient settings for point-of-care testing, whereas determinations of PSA (e.g., PSA-T, PSA-F, or PSA-ACT) performed within the time frame of the office visit would provide results equivalent to conventional analyses performed in serum.

The following articles in journals at HighWire Press have cited this article:

				Q.-P. Qin, M. Christiansen, and K. Pettersson Point-of-Care Time-resolved Immunofluorometric Assay for Human Pregnancy-associated Plasma Protein A: Use in First-Trimester Screening for Down Syndrome Clin. Chem., March 1, 2002; 48(3): 473 - 483. [Abstract] [Full Text] [PDF]

				T. A. Stamey Preoperative Serum Prostate-specific Antigen (PSA) Below 10 {micro}g/L Predicts Neither the Presence of Prostate Cancer Nor the Rate of Postoperative PSA Failure Clin. Chem., April 1, 2001; 47(4): 631 - 634. [Abstract] [Full Text] [PDF]