Clinical Chemistry Link to Randox Laboratories Web Site
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 48: 613-621, 2002;
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Data
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (34)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bjerner, J.
Right arrow Articles by Børmer, O. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bjerner, J.
Right arrow Articles by Børmer, O. P.
Related Collections
Right arrow Proteomics and Protein Markers
(Clinical Chemistry. 2002;48:613-621.)
© 2002 American Association for Clinical Chemistry, Inc.

Immunometric Assay Interference

Incidence and Prevention

Johan Bjerner1a, Kjell Nustad1, Lars F. Norum1, Kari Hauge Olsen1 and Ole P. Børmer1

1 Central Laboratory, Norwegian Radium Hospital, Montebello, N-0310 Oslo, Norway.

aAuthor for correspondence. Fax 47-22-730725; e-mail johan.bjerner{at}klinmed.uio.no.

Background: The primary aim of the study was to reduce interference in an in-house two-site, two-step immunometric assay.

Methods: In the running laboratory routine, 11 261 samples were tested with a carcinoembryonic antigen (CEA) assay with bovine immunoglobulin but no murine immunoglobulins in the buffer, in parallel to our routine CEA assay, using 15 mg/L heat-treated nonspecific murine immunoglobulin (MAK33) in the buffer and with the Fc fragments removed from the capture antibody.

Results: The frequency of interference was estimated to be 4.0% (95% confidence interval, 3.3–4.7%). The addition of 15 mg/L native MAK33 had little effect (frequency, 3.9%; 95% confidence interval, 3.2–4.6%), whereas adding 15 mg/L heat-treated MAK33 reduced interference to 0.86% (0.61–1.12%), and adding 50 mg/L reduced it further to 0.06% (0–0.13%). Removing the Fc fragments by itself reduced interference to 0.10% (0.02–0.19%). There were no statistically significant differences for age (P <0.23) or gender (P <0.40) between patients with interference (n = 210) and a randomly selected interference-negative control group (n = 186). Interference was not constant in patients: 15 of 25 individuals positive for interference and with four or more samples screened for interference had an interference-negative sample either before or after the peak of interference.

Conclusions: In a two-site, two-step immunometric assay using mouse monoclonal antibodies, use of heat-treated nonspecific murine immunoglobulin in the buffer or removal of the Fc fragment from the capture antibody could improve performance.




The following articles in journals at HighWire Press have cited this article:


Home page
 Clin. Chem.Home page
M. S. Nordlund, D. J. Warren, K. Nustad, J. Bjerner, and E. Paus
Automated Time-Resolved Immunofluorometric Assay for Progastrin-Releasing Peptide
Clin. Chem., May 1, 2008; 54(5): 919 - 922.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
U.-H. Stenman
Improving Immunoassay Performance by Antibody Engineering
Clin. Chem., May 1, 2005; 51(5): 801 - 802.
[Full Text] [PDF]

Home page
 Clin. Chem.Home page
D. J. Warren, J. Bjerner, E. Paus, O. P. Bormer, and K. Nustad
Use of an In Vivo Biotinylated Single-Chain Antibody as Capture Reagent in an Immunometric Assay to Decrease the Incidence of Interference from Heterophilic Antibodies
Clin. Chem., May 1, 2005; 51(5): 830 - 838.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
J. Bjerner, O. P. Bormer, and K. Nustad
The War on Heterophilic Antibody Interference
Clin. Chem., January 1, 2005; 51(1): 9 - 11.
[Full Text] [PDF]

Home page
 Clin. Chem.Home page
C. M. Preissner, L. A. Dodge, D. J. O'Kane, R. J. Singh, and S. K.G. Grebe
Prevalence of Heterophilic Antibody Interference in Eight Automated Tumor Marker Immunoassays
Clin. Chem., January 1, 2005; 51(1): 208 - 210.
[Full Text] [PDF]

Home page
 Clin. Chem.Home page
A. A.A. Ismail
A Radical Approach Is Needed to Eliminate Interference from Endogenous Antibodies in Immunoassays
Clin. Chem., January 1, 2005; 51(1): 25 - 26.
[Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
C. M. Preissner, D. J. O'Kane, R. J. Singh, J. C. Morris, and S. K. G. Grebe
Phantoms in the Assay Tube: Heterophile Antibody Interferences in Serum Thyroglobulin Assays
J. Clin. Endocrinol. Metab., July 1, 2003; 88(7): 3069 - 3074.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
U.-H. Stenman
Standardization as a Private Enterprise
Clin. Chem., April 1, 2003; 49(4): 535 - 536.
[Full Text] [PDF]

Home page
 Clin. Chem.Home page
A. A.A. Ismail, P. L. Walker, J. H. Barth, K. C. Lewandowski, R. Jones, and W. A. Burr
Wrong Biochemistry Results: Two Case Reports and Observational Study in 5310 Patients on Potentially Misleading Thyroid-stimulating Hormone and Gonadotropin Immunoassay Results
Clin. Chem., November 1, 2002; 48(11): 2023 - 2029.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2002 by the American Association for Clinical Chemistry.