Clinical Chemistry AACC Online Job Center
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 49: 32-40, 2003; 10.1373/49.1.32
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (119)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Auerbach, R.
Right arrow Articles by Akhtar, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Auerbach, R.
Right arrow Articles by Akhtar, N.
Related Collections
Right arrow Other Areas of Clinical Chemistry
(Clinical Chemistry. 2003;49:32-40.)
© 2003 American Association for Clinical Chemistry, Inc.

Overview

Angiogenesis Assays: A Critical Overview

Robert Auerbacha1, Rachel Lewis1, Brenda Shinners1, Louis Kubai1 and Nasim Akhtar1

1 Laboratory of Developmental Biology, University of Wisconsin, 1117 West Johnson St., Madison, WI 53706.

aAuthor for correspondence. Fax 608-262-1171; e-mail rauerbac{at}facstff.wisc.edu.


Abstract

Background: Angiogenesis, the formation of new blood vessels, is an integral part of both normal developmental processes and numerous pathologies, ranging from tumor growth and metastasis to inflammation and ocular disease. Angiogenesis assays are used to test efficacy of both pro- and antiangiogenic agents.

Methods: Most studies of angiogenesis inducers and inhibitors rely on various models, both in vitro and in vivo, as indicators of efficacy. In this report we describe the principal methods now in use: the in vivo Matrigel plug and corneal neovascularization assays, the in vivo/in vitro chick chorioallantoic membrane (CAM) assay, and the in vitro cellular (proliferation, migration, tube formation) and organotypic (aortic ring) assays. We include description of two new methods, the chick aortic arch and the Matrigel sponge assays.

Conclusions: In vitro tests are valuable, can be carried out expeditiously, and lend themselves to quantification, but must be interpreted with extreme caution. In vitro tests are best viewed as providing initial information, subject to confirmation by in vivo assays. Multiple tests should be used to obtain maximum benefit from in vitro tests. In vivo tests are more difficult and time-consuming to perform, thereby limiting the number of tests that can run at any one time. Quantification is generally more difficult as well. However, in vivo assays are essential because of the complex nature of vascular responses to test reagents, responses that no in vitro model can fully achieve.




The following articles in journals at HighWire Press have cited this article:


Home page
 Am. J. Pathol.Home page
J. S. Blackburn and C. E. Brinckerhoff
Matrix Metalloproteinase-1 and Thrombin Differentially Activate Gene Expression in Endothelial Cells via PAR-1 and Promote Angiogenesis
Am. J. Pathol., December 1, 2008; 173(6): 1736 - 1746.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Chen, Y. C. Levine, D. E. Golan, T. Michel, and A. J. Lin
Atrial Natriuretic Peptide-initiated cGMP Pathways Regulate Vasodilator-stimulated Phosphoprotein Phosphorylation and Angiogenesis in Vascular Endothelium
J. Biol. Chem., February 15, 2008; 283(7): 4439 - 4447.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
U. Tigges, E. G. Hyer, J. Scharf, and W. B. Stallcup
FGF2-dependent neovascularization of subcutaneous Matrigel plugs is initiated by bone marrow-derived pericytes and macrophages
Development, February 1, 2008; 135(3): 523 - 532.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
S. Schwartz, J. George, J. Ben-Shoshan, G. Luboshits, I. Avni, H. Levkovitch-Verbin, H. Ziv, M. Rosner, and A. Barak
Drug Modification of Angiogenesis in a Rat Cornea Model
Invest. Ophthalmol. Vis. Sci., January 1, 2008; 49(1): 250 - 254.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
S. J. Netherton, J. A. Sutton, L. S. Wilson, R. L. Carter, and D. H. Maurice
Both Protein Kinase A and Exchange Protein Activated by cAMP Coordinate Adhesion of Human Vascular Endothelial Cells
Circ. Res., October 12, 2007; 101(8): 768 - 776.
[Abstract] [Full Text] [PDF]

Home page
 Molecular Cancer TherapeuticsHome page
B. Martinez-Poveda, R. Munoz-Chapuli, S. Rodriguez-Nieto, J. M. Quintela, A. Fernandez, M.-A. Medina, and A. R. Quesada
IB05204, a dichloropyridodithienotriazine, inhibits angiogenesis in vitro and in vivo
Mol. Cancer Ther., October 1, 2007; 6(10): 2675 - 2685.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. C. Levine, G. K. Li, and T. Michel
Agonist-modulated Regulation of AMP-activated Protein Kinase (AMPK) in Endothelial Cells: EVIDENCE FOR AN AMPK -> Rac1 -> Akt -> ENDOTHELIAL NITRIC-OXIDE SYNTHASE PATHWAY
J. Biol. Chem., July 13, 2007; 282(28): 20351 - 20364.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
A. Saito, A. Sugawara, A. Uruno, M. Kudo, H. Kagechika, Y. Sato, Y. Owada, H. Kondo, M. Sato, M. Kurabayashi, et al.
All-trans Retinoic Acid Induces in Vitro Angiogenesis via Retinoic Acid Receptor: Possible Involvement of Paracrine Effects of Endogenous Vascular Endothelial Growth Factor Signaling
Endocrinology, March 1, 2007; 148(3): 1412 - 1423.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. C. Lake, R. Vassy, M. Di Benedetto, D. Lavigne, C. Le Visage, G. Y. Perret, and D. Letourneur
Low Molecular Weight Fucoidan Increases VEGF165-induced Endothelial Cell Migration by Enhancing VEGF165 Binding to VEGFR-2 and NRP1
J. Biol. Chem., December 8, 2006; 281(49): 37844 - 37852.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
L. A. Kunz-Schughart, J. A. Schroeder, M. Wondrak, F. van Rey, K. Lehle, F. Hofstaedter, and D. N. Wheatley
Potential of fibroblasts to regulate the formation of three-dimensional vessel-like structures from endothelial cells in vitro
Am J Physiol Cell Physiol, May 1, 2006; 290(5): C1385 - C1398.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
D. A. Glesne, W. Zhang, S. Mandava, L. Ursos, M. E. Buell, L. Makowski, and D. J. Rodi
Subtractive Transcriptomics: Establishing Polarity Drives In vitro Human Endothelial Morphogenesis.
Cancer Res., April 15, 2006; 66(8): 4030 - 4040.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
S. I. Zittermann and A. C. Issekutz
Basic Fibroblast Growth Factor (bFGF, FGF-2) Potentiates Leukocyte Recruitment to Inflammation by Enhancing Endothelial Adhesion Molecule Expression
Am. J. Pathol., March 1, 2006; 168(3): 835 - 846.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Bohman, T. Matsumoto, K. Suh, A. Dimberg, L. Jakobsson, S. Yuspa, and L. Claesson-Welsh
Proteomic Analysis of Vascular Endothelial Growth Factor-induced Endothelial Cell Differentiation Reveals a Role for Chloride Intracellular Channel 4 (CLIC4) in Tubular Morphogenesis
J. Biol. Chem., December 23, 2005; 280(51): 42397 - 42404.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
P.-L. Tremblay, F. Berthod, L. Germain, and F. A. Auger
In Vitro Evaluation of the Angiostatic Potential of Drugs Using an Endothelialized Tissue-Engineered Connective Tissue
J. Pharmacol. Exp. Ther., November 1, 2005; 315(2): 510 - 516.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
M. Rajesh, A. Kolmakova, and S. Chatterjee
Novel Role of Lactosylceramide in Vascular Endothelial Growth Factor-Mediated Angiogenesis in Human Endothelial Cells
Circ. Res., October 14, 2005; 97(8): 796 - 804.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
R. Jarrahy, W. Huang, G. H. Rudkin, J. M. Lee, K. Ishida, M. D. Berry, M. Sukkarieh, B. M. Wu, D. T. Yamaguchi, and T. A. Miller
Osteogenic differentiation is inhibited and angiogenic expression is enhanced in MC3T3-E1 cells cultured on three-dimensional scaffolds
Am J Physiol Cell Physiol, August 1, 2005; 289(2): C408 - C414.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
B. W. Ennis, K. E. Fultz, K. A. Smith, J. K. Westwick, D. Zhu, M. Boluro-Ajayi, G. K. Bilter, and B. Stein
Inhibition of Tumor Growth, Angiogenesis, and Tumor Cell Proliferation by a Small Molecule Inhibitor of c-Jun N-terminal Kinase
J. Pharmacol. Exp. Ther., April 1, 2005; 313(1): 325 - 332.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
D. A. Ingram, L. E. Mead, D. B. Moore, W. Woodard, A. Fenoglio, and M. C. Yoder
Vessel wall-derived endothelial cells rapidly proliferate because they contain a complete hierarchy of endothelial progenitor cells
Blood, April 1, 2005; 105(7): 2783 - 2786.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
S. J. Netherton and D. H. Maurice
Vascular Endothelial Cell Cyclic Nucleotide Phosphodiesterases and Regulated Cell Migration: Implications in Angiogenesis
Mol. Pharmacol., January 1, 2005; 67(1): 263 - 272.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
A. Martinez, E. Zudaire, S. Portal-Nunez, L. Guedez, S. K. Libutti, W. G. Stetler-Stevenson, and F. Cuttitta
Proadrenomedullin NH2-Terminal 20 Peptide Is a Potent Angiogenic Factor, and Its Inhibition Results in Reduction of Tumor Growth
Cancer Res., September 15, 2004; 64(18): 6489 - 6494.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
F. MacKenzie, P. Duriez, B. Larrivee, L. Chang, I. Pollet, F. Wong, C. Yip, and A. Karsan
Notch4-induced inhibition of endothelial sprouting requires the ankyrin repeats and involves signaling through RBP-J{kappa}
Blood, September 15, 2004; 104(6): 1760 - 1768.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Zijlstra, R. T. Aimes, D. Zhu, K. Regazzoni, T. Kupriyanova, M. Seandel, E. I. Deryugina, and J. P. Quigley
Collagenolysis-dependent Angiogenesis Mediated by Matrix Metalloproteinase-13 (Collagenase-3)
J. Biol. Chem., June 25, 2004; 279(26): 27633 - 27645.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
W. E. G. Muller, N. L. Thakur, H. Ushijima, A. N. Thakur, A. Krasko, G. Le Pennec, M. M. Indap, S. Perovic-Ottstadt, H. C. Schroder, G. Lang, et al.
Matrix-mediated canal formation in primmorphs from the sponge Suberites domuncula involves the expression of a CD36 receptor-ligand system
J. Cell Sci., May 15, 2004; 117(12): 2579 - 2590.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
D. S. Kaufman, R. L. Lewis, E. T. Hanson, R. Auerbach, J. Plendl, and J. A. Thomson
Functional endothelial cells derived from rhesus monkey embryonic stem cells
Blood, February 15, 2004; 103(4): 1325 - 1332.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
H. Zheng, C. Wasylyk, A. Ayadi, J. Abecassis, J. A Schalken, H. Rogatsch, N. Wernert, S.-M. Maira, M.-C. Multon, and B. Wasylyk
The transcription factor Net regulates the angiogenic switch
Genes & Dev., September 15, 2003; 17(18): 2283 - 2297.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2003 by the American Association for Clinical Chemistry.