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Gold-Labeled Nanoparticle-Based Immunoresonance Scattering Spectral Assay for Trace Apolipoprotein AI and Apolipoprotein B

¹ Department of Resource and Environmental Science, Guangxi Normal University, Guilin, China.
² Department of Material and Chemical Engineering, Guilin University of Technology, Guilin, China.

^aAddress correspondence to this author at: Department of Resource and Environmental Science, Guangxi Normal University, Guilin 541004, China.

Background: Apolipoprotein AI (ApoAI) and ApoB are risk indicators of cardiovascular disease. We describe the use of immunoresonance scattering to measure the ApoAI and ApoB in serum.

Methods: We used a trisodium citrate method to prepare 9.0-nm gold nanoparticles labeled with goat anti-human ApoAI and ApoB antibodies. The immune reaction between gold-labeled antibodies and antigens took place in Na₂HPO₄-NaH₂PO₄ buffer solution (pH 6.4 for ApoAI and pH 6.0 for ApoB) in the presence of 75 g/L polyethylene glycol (PEG). We used a transmission electron microscope to observe the shape of the gold nanoparticles. Results were compared with those obtained by immunoturbidimetric methods. Twenty-five human serum samples were assayed by the immunoresonance scattering assay preset with the data indicated and by an immunoturbidimetric assay.

Results: The presence of PEG greatly enhanced the intensity of resonance-scattering peaks at 560 nm. The intensity (I) was proportional to concentration at 0.00833–0.3333 mg/L ApoAI and 0.00197–0.1972 mg/L ApoB. The detection limits were 2.04 and 0.96 µg/L for ApoAI and ApoB, respectively. The results for human serum samples were in agreement with those obtained with an immunoturbidimetric method. Linear regression analysis revealed a correlation coefficient, slope, and intercept of 0.915, 0.966, and 68.53 mg/L, respectively, for ApoAI and 0.919, 0.996, and 15.46 mg/L for ApoB.

Conclusion: This method showed high sensitivity and good selectivity for quantitative determination of ApoAI and ApoB in human serum, with satisfactory results.

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				Z. Jiang, L. Wei, M. Zou, A. Liang, and M. Meng Rapid Assay of Trace Immunoglobulin M by a New Immunonanogold Resonance Scattering Spectral Probe J Biomol Screen, April 1, 2008; 13(4): 302 - 308. [Abstract] [PDF]

				Z. Jiang, W. Huang, J. Li, M. Li, A. Liang, S. Zhang, and B. Chen Nanogold Catalysis Based Immunoresonance-Scattering Spectral Assay for Trace Complement Component 3 Clin. Chem., January 1, 2008; 54(1): 116 - 123. [Abstract] [Full Text] [PDF]