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Clinical Chemistry 53: 2042-2050, 2007. First published October 11, 2007; 10.1373/clinchem.2007.091488
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(Clinical Chemistry. 2007;53:2042-2050.)
© 2007 American Association for Clinical Chemistry, Inc.

Molecular Diagnostics and Genetics

Tentacle ProbesTM: Differentiation of Difficult Single-Nucleotide Polymorphisms and Deletions by Presence or Absence of a Signal in Real-Time PCR

Brent C. Satterfield1,2, David A. Kulesh3, David A. Norwood3, Leonard P. Wasieloski, Jr3, Michael R. Caplan1,a and Jay A.A. West2

1 Harrington Department of Bioengineering, Arizona State University, Tempe, AZ.
2 Arcxis Biotechnologies, Pleasanton, CA.
3 United States Army Medical Research Institute of Infectious Diseases, Frederick, MD.

aAddress correspondence to this author at: Arizona State University, PO Box 879709, Tempe, AZ 85287-9709. Fax 480-727-7624; e-mail Michael.Caplan{at}asu.edu.

Background: False-positive results are a common problem in real-time PCR identification of DNA sequences that differ from near neighbors by a single-nucleotide polymorphism (SNP) or deletion. Because of a lack of sufficient probe specificity, post-PCR analysis, such as a melting curve, is often required for mutation differentiation.

Methods: Tentacle ProbesTM, cooperative reagents with both a capture and a detection probe based on specific cell-targeting principles, were developed as a replacement for 2 chromosomal TaqMan–minor groove binder (MGB) assays previously developed for Yersinia pestis and Bacillus anthracis detection. We compared TaqMan-MGB probes to Tentacle Probes for SNP and deletion detection based on the presence or absence of a growth curve.

Results: With the TaqMan-MGB Y. pestis yp48 assays, false-positive results for Yersinia pseudotuberculosis occurred at every concentration tested, and with the TaqMan-MGB B. anthracis gyrA assays, false-positive results occurred in 21 of 29 boil preps of environmental samples of near neighbors. With Tentacle Probes no false-positive results occurred.

Conclusions: The high specificity exhibited by Tentacle Probes may eliminate melting curve analysis for SNP and deletion mutation detection, allowing the diagnostic use of previously difficult targets.




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Home page
 Nucleic Acids ResHome page
B. C. Satterfield, M. R. Caplan, and J. A. A. West
Tentacle probe sandwich assay in porous polymer monolith improves specificity, sensitivity and kinetics
Nucleic Acids Res., September 12, 2008; (2008) gkn564v1.
[Abstract] [Full Text] [PDF]


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Copyright © 2007 by the American Association for Clinical Chemistry.