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Clinical Chemistry 6: 269-275, 1960;
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Clinical Chemistry, Vol 6, 269-275, Copyright © 1960 by the American Association for Clinical Chemistry

Rapid Method for the Quantitative Determination of Serum Alkaline Phosphatase

Bernard klein 1, Prunella A. Read 1, and Arthur L. Babson 1

1 Laboratory Service, Veterans Administration Hospital, Bronx, N. Y., and The Warner-Lambert Research Institute, Morris Plains, N. J.

A new procedure is described for the simple, rapid, quantitative measurement of serum alkaline phosphatase. The procedure is based on the direct photometric measurement of phenolphthalein released from sodium phenolphthalein phosphate. The substrate is available in a convenient, stabilized form as a tablet containing buffer and substrate in optimum amounts for a single analysis. As many as 30 analyses can be performed in an hour.




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