Clinical Chemistry, Vol 6, 413-420, Copyright © 1960 by the American Association for Clinical Chemistry

Nonionic Surfactants in Paper Electrophoresis

¹ Midwest Medical Research foundation, 3241 Victor Place, Wichita 8, Kan.

1. A modification of the usual paper electrophoretic procedure has been made by including in the buffer medium 0.05 per cent of certain alkylphenyl ethers of polyethylene glycol.

2. This modified procedure yields electrophoretic patterns of blood serum proteins that may be subdivided into 12 fractions— albumin, ₁-globulin, three ₂-globulins, three -globulins, and four -globulins.

3. The method was applied to blood serum from 70 patients apparently free of significant disease and to sera from a number of patients with varied disease. More specific location of the altered protein levels was possible in a number of disease states.

4. Glycoprotein distribution was not seriously disturbed by the presence of the nonionic surfactants, but lipoproteins migrated at a decreased rate.