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Clinical Chemistry 7: 275-284, 1961;
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Clinical Chemistry, Vol 7, 275-284, Copyright © 1961 by the American Association for Clinical Chemistry

A Method for Measurement of Cholesterol in Blood Serum

George V. Mann 1

1 Departments of Surgery, Biochemistry and Medicine, Vanderbilt University School of Medicine, Nashville, Tenn.

A method for measuring cholesterol in 0.1 ml. of blood serum is described. This uses the saponification and extraction procedure of Abell et al. (4) and the color reaction of Zlatkis et al. (7). The procedure is shown to be accurate and reproducible. It is compared with several commonly used procedures and shown to be equal or superior to these. An adaptation of the method to filter-paper stored samples described. The method is recommended for use in clinical laboratories.

Submitted on November 17, 1960




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