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Clinical Chemistry 8: 166-171, 1962;
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Clinical Chemistry, Vol 8, 166-171, Copyright © 1962 by the American Association for Clinical Chemistry

Micro Immunochemical Method for Estimating High- and Low-Density Lipoproteins

R. L. Searcy 1, V. P. Carroll Jr. 1, J. S. Carlucci 1, and L. M. Bergquist 1

1 Departments of Pathology, Los Angeles County General Hospital, Unit 2, California College of Medicine, Los Angeles, Calif.

A rapid technic is proposed for immunochemically measuring total, high- and low-density lipoprotein cholesterol as well as total low-density lipoprotein precipitates in small quantities of human serum. Lipoprotein cholesterol values obtained by the method are reproducible and correlate well with those yielded by paper electrophoresis. The immunochemical procedure has been used to assess serum lipid fractions in 10 patients during a short-term course of sodium dextro-thyroxine therapy.

Submitted on May 26, 1961




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