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Clinical Chemistry 8: 215-235, 1962;
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Clinical Chemistry, Vol 8, 215-235, Copyright © 1962 by the American Association for Clinical Chemistry

An o-Toluidine Method for Body-Fluid Glucose Determination

Kurt M. Dubowski 1

1 Clinical Chemistry and Toxicology Laboratories, University of Florida Teaching Hospital and Clinics, Gainesville, Fla.

o-Toluidine, 6% (v/v) in glacial acetic acid, is used to determine glucose in biologic material after deproteinization with 3% (w/v) trichloracetic acid. A stable green color develops after heating at 100° for 10 min., and the absorbance is determined at 630 or 635 mµ. The reagent is stable for many months at room temperature, and the reaction follows Beer's Law over a very wide range of concentrations. The development of the procedure is discussed, as is the specificity of the method for glucose.

Submitted on July 6, 1961




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