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Clinical Chemistry 8: 509-519, 1962;
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Clinical Chemistry, Vol 8, 509-519, Copyright © 1962 by the American Association for Clinical Chemistry

Serum Lipase Determination with an Olive Oil Substrate Using a Three-Hour Incubation Period

Roderick P. MacDonald 1 and Royal O. LeFave 1

1 Department of Pathology, Harper Hospital, Detroit, Mich.

The effect of various emulsifying agents, pH, buffers, and accelerators on the Cherry-Crandall procedure for determining serum lipase was studied. A method is proposed which uses a 3-hour incubation period. Olive oil emulsified with 7% acacia is the substrate. This is buffered with tris buffer, pH 8.0; magnesium acetate and EDTA are added as accelerators. Other conditions which result in an increased rate of hydrolysis are discussed.

Submitted on October 6, 1961






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Copyright © 1962 by the American Association for Clinical Chemistry.