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Clinical Chemistry 8: 606-615, 1962;
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Clinical Chemistry, Vol 8, 606-615, Copyright © 1962 by the American Association for Clinical Chemistry

Specific Enzymatic Determination of Glucose in Blood Serum or Plasma by an Automatic Potentiometric Reaction-Rate Method

H. V. Malmstadt 1 and H. L. Pardue 1

1 Department of Chemistry and Chemical Engineering, University of Illinois, Urbana, Ill.

A new automatic potentiometric reaction-rate method has been applied to the specific enzymatic measurement of glucose in blood plasma or serum. A new filtering technic is described for removal of precipitated protein. The use of injection pipets to simplify and speed up the reagent-and sample-handling step is described. Glucose is determined in 0.02 ml. of serum or plasma with relative errors within 2%. The average measurement time is about 30 sec.

Submitted on August 21, 1961






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Copyright © 1962 by the American Association for Clinical Chemistry.