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Clinical Chemistry 9: 156-162, 1963;
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Clinical Chemistry, Vol 9, 156-162, Copyright © 1963 by the American Association for Clinical Chemistry

Errors Associated with the Direct Measurement of Human Serum Cholesterol Using the FeCI3 Color Reagent

Robert V. Moore 1 and Edwin Boyle Jr. 1

1 Lipid Metabolism Laboratory, Department of Medicine, Medical College of South Carolina, Charleston, S. C.

Evidence is presented concerning errors in the direct determination of human serum cholesterol with a ferric-chloride color reagent. It is shown that in physiologic concentrations the vitamins A, D, E, K, and niotinic acid cause no error, but that hemoglobin, anticoagulants, and impurities in acetic acid can cause errors. The control of these errors is discussed. Errors caused by steroids, added salts, and high bilirubin content of serum are discussed.

Submitted on November 2, 1961






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Copyright © 1963 by the American Association for Clinical Chemistry.