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Clinical Chemistry 9: 168-181, 1963;
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Clinical Chemistry, Vol 9, 168-181, Copyright © 1963 by the American Association for Clinical Chemistry

A Rapid and Sensitive Turbidimetric Method for Serum Lipase Based Upon Differences Between the Lipases of Normal and Pancreatitis Serum

William C. Vogel 1 and Leslie Zieve 1

1 Radioisotope Service and Department of Medicine, Veterans Administration Hospital, University of Minnesota, Minneapolis 17, Minn.

A rapid turbidimetric method for the determination of serum lipase is described. The substrate is a nearly pure triglyceride in Tris buffer, with sodium deoxycholate as the emulsifying agent. The method is equal in sensitivity to the titrimetric measure of serum lipase using a modified Cherry-Crandall substrate.

The serum of patients with acute pancreatitis contains a lipase with optimal action at pH 9.1 and sodium deoxycholate concentration of 0.35%. These are different from the optimal pH and bile salt requirement for lipase activity in normal serum or duodenal contents.

Submitted on November 30, 1961




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