Clinical Chemistry Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 43: 2186-2187, 1997;
This Article
Right arrow Full Text
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (4)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fermo, I.
Right arrow Articles by Paroni, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fermo, I.
Right arrow Articles by Paroni, R.
Related Collections
Right arrow Endocrinology and Metabolism
(Clinical Chemistry. 1997;43:2186-2187.)
© 1997 American Association for Clinical Chemistry, Inc.

Technical Briefs

Urine Pyridinium Cross-Links Determination by Beckman Cross Links Kit

Isabella Fermoa, Cinzia Arcelloni, Erminia Casari1 and Rita Paroni

1 Ist. Clin. Humanitas, Rozzano (MI), Italy;
a address for correspondence: Lab. HPLC, H. San Raffaele, Via Olgettina 60, 20132 Milano, Italy, fax 39-2-26432640

Increased understanding of bone turnover has led to the development of several biochemical tests of bone metabolism. Among the biochemical indexes of bone resorption is measurement of urinary excretion of pyridinoline (Pyr) and deoxypyridinoline (Dpyr), molecules that cross-link the collagen chains and are released into the systemic circulation after the breakdown of mature bone collagen (1)(2)(3). Because they are not metabolized in vivo, they are excreted directly into urine in free (~40%) and peptide-bound forms (~60%) (4). Methods to measure cross-links in urine involve mainly two technical approaches: HPLC analysis, which, after hydrolytic and purification steps, allows the determination of the total forms of cross-links (5)(6)(7), and monoclonal antibody immunoassay methods able to quantify the sum of free Pyr and Dpyr or only the free Dpyr form (8)(9). The quantification of total or free cross-links forms provides, in any case, similar clinical information (9)(10).

Here we report the evaluation of the Cross LinksTM HPLC kit recently introduced by Beckman Labs. to quantify the total forms of Pyr and Dpyr. We compared the performance . . . [Full Text of this Article]


Acknowledgments


Footnotes


References




The following articles in journals at HighWire Press have cited this article:


Home page
 Cancer Epidemiol. Biomarkers Prev.Home page
Y.-C. Chung, C.-H. Ku, T.-Y. Chao, J.-C. Yu, M. M. Chen, and S.-H. Lee
Tartrate-resistant Acid phosphatase 5b activity is a useful bone marker for monitoring bone metastases in breast cancer patients after treatment.
Cancer Epidemiol. Biomarkers Prev., March 1, 2006; 15(3): 424 - 428.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
T. G. Rosano, R. T. Peaston, H. G. Bone, H. W. Woitge, R. M. Francis, and M. J. Seibel
Urinary free deoxypyridinoline by chemiluminescence immunoassay: analytical and clinical evaluation
Clin. Chem., October 1, 1998; 44(10): 2126 - 2132.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1997 by the American Association for Clinical Chemistry.