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Clinical Chemistry 43: 405-406, 1997;
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(Clinical Chemistry. 1999;43:405-406.)
© 1999 American Association for Clinical Chemistry, Inc.

Technical Briefs

Quantitative Immunological Detection of Total Estrogen Receptor (Cytosolic and Nuclear) in Term Decidua of Preeclampsia: a Preliminary Study

Sanaa Eissa1,a, Mohamed M. Mostafa2, Alaa A. E. A. El-Gendy3 and Ibrahim A. Senna

1 Oncology Diagnostic Unit, Biochem. Dept.,
2 Dept. of Gyn., Ain Shams Faculty of Med., Abbassia, Cairo, Egypt, and
3 Fayoum General Hospital, Egypt;
a address for correspondence: fax 202-285-9928

Although progesterone and estrogens are essential for maintaining human pregnancy after implantation, few reports have investigated the localization of their specific receptors in different uterine cell types throughout pregnancy (1)(2). Wu et al. detected estrogen receptors (ER) in low concentrations in decidua in early pregnancy but not in term decidua (2). ER concentrations in term decidua of pregnancies with complications (in particular, preeclampsia) have not been investigated.

Classical biochemical assay methods estimate ER either in cytosol fraction or in the high-salt extracted nuclei (3)(4)(5). These assays need two subcellular fractionation steps and two ER assays in two fractions: cytosol and nuclear extract. To measure total ER (cytosolic and nuclear) in one fraction using one single assay in decidual samples, we modified the assay method by using a homogenization buffer containing KCl. The effective KCl concentration for maximum ER extraction without inhibition of the ER . . . [Full Text of this Article]


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Copyright © 1997 by the American Association for Clinical Chemistry.