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Technical Briefs |
1
Div. of Med. Genetics,
2
Div. of Clin. Biochem., Royal Victoria Hosp., 687 Pine Ave. W., Montreal, QC H3A 1A1, Canada;
Homocysteine (Hcy), increasingly being recognized as a risk factor for vascular disease (1), is found primarily in plasma in the form of homocystine and mixed disulfides, both protein-bound and unbound; total Hcy (tHcy) is the sum of all Hcy species obtained after quantitative reduction. Several methods are currently used to measure tHcy in plasma, including GC/MS, ion-exchange chromatography, and HPLC (2). In one of the most popular HPLC techniques, any homocystine and homocysteine-mixed disulfides present are reduced with tri-butyl phosphine (TBP) to Hcy, which is subsequently derivatized with ammonium 7-fluorobenzo-2-oxa-1,3-diazole-4-sulfonate (SBD-F) to produce a fluorescent product (3)(4).
TBP has a highly disagreeable odor and is poorly soluble in water so
that it must be dissolved in dimethylformamide for use, both of which
properties hinder the routine use of TBP in a clinical laboratory.
Here, we call attention to a
Acknowledgments
Footnotes
References
The following articles in journals at HighWire Press have cited this article:
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  M. G. Garrod, R. Green, L. H. Allen, D. M. Mungas, W. J. Jagust, M. N. Haan, and J. W. Miller Fraction of Total Plasma Vitamin B12 Bound to Transcobalamin Correlates with Cognitive Function in Elderly Latinos with Depressive Symptoms Clin. Chem., July 1, 2008; 54(7): 1210 - 1217. [Abstract] [Full Text] [PDF]
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 F. Simard, F. Guay, C. L. Girard, A. Giguere, J.-P. Laforest, and J. J. Matte Effects of concentrations of cyanocobalamin in the gestation diet on some criteria of vitamin B12 metabolism in first-parity sows J Anim Sci, December 1, 2007; 85(12): 3294 - 3302. [Abstract] [Full Text] [PDF]
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 E. D. Louis, N. Schupf, M. X. Tang, K. Marder, and J. A. Luchsinger Mild Parkinsonian Signs and Plasma Homocysteine Concentration in Community-Dwelling Elderly Individuals Arch Neurol, November 1, 2007; 64(11): 1646 - 1651. [Abstract] [Full Text] [PDF]
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 M. Xie, S. S. Hou, W. Huang, and H. P. Fan Effect of Excess Methionine and Methionine Hydroxy Analogue on Growth Performance and Plasma Homocysteine of Growing Pekin Ducks Poult. Sci., September 1, 2007; 86(9): 1995 - 1999. [Abstract] [Full Text] [PDF]
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 M. N Haan, J. W Miller, A. E Aiello, R. A Whitmer, W. J Jagust, D. M Mungas, L. H Allen, and R. Green Homocysteine, B vitamins, and the incidence of dementia and cognitive impairment: results from the Sacramento Area Latino Study on Aging Am. J. Clinical Nutrition, February 1, 2007; 85(2): 511 - 517. [Abstract] [Full Text] [PDF]
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J. W. Miller, M. G. Garrod, A. L. Rockwood, M. M. Kushnir, L. H. Allen, M. N. Haan, and R. Green Measurement of Total Vitamin B12 and Holotranscobalamin, Singly and in Combination, in Screening for Metabolic Vitamin B12 Deficiency Clin. Chem., February 1, 2006; 52(2): 278 - 285. [Abstract] [Full Text] [PDF]
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M. I Ramos, L. H Allen, D. M Mungas, W. J Jagust, M. N Haan, R. Green, and J. W Miller Low folate status is associated with impaired cognitive function and dementia in the Sacramento Area Latino Study on Aging Am. J. Clinical Nutrition, December 1, 2005; 82(6): 1346 - 1352. [Abstract] [Full Text] [PDF]
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 C. Papoutsakis, N. Yiannakouris, Y. Manios, E. Papaconstantinou, F. Magkos, K. H. Schulpis, A. Zampelas, and A. L. Matalas Plasma Homocysteine Concentrations in Greek Children Are Influenced by an Interaction between the Methylenetetrahydrofolate Reductase C677T Genotype and Folate Status J. Nutr., March 1, 2005; 135(3): 383 - 388. [Abstract] [Full Text] [PDF]
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M. I Ramos, L. H Allen, M. N Haan, R. Green, and J. W Miller Plasma folate concentrations are associated with depressive symptoms in elderly Latina women despite folic acid fortification Am. J. Clinical Nutrition, October 1, 2004; 80(4): 1024 - 1028. [Abstract] [Full Text] [PDF]
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Y. Lin, S. R Dueker, J. R Follett, J. G Fadel, A. Arjomand, P. D Schneider, J. W Miller, R. Green, B. A Buchholz, J. S Vogel, et al. Quantitation of in vivo human folate metabolism Am. J. Clinical Nutrition, September 1, 2004; 80(3): 680 - 691. [Abstract] [Full Text] [PDF]
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 J. A. Luchsinger, M. -X. Tang, S. Shea, J. Miller, R. Green, and R. Mayeux Plasma homocysteine levels and risk of Alzheimer disease Neurology, June 8, 2004; 62(11): 1972 - 1976. [Abstract] [Full Text] [PDF]
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A. K. Shoveller, J. D. House, J. A. Brunton, P. B. Pencharz, and R. O. Ball The Balance of Dietary Sulfur Amino Acids and the Route of Feeding Affect Plasma Homocysteine Concentrations in Neonatal Piglets J. Nutr., March 1, 2004; 134(3): 609 - 612. [Abstract] [Full Text] [PDF]
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A. K. Shoveller, J. A. Brunton, J. D. House, P. B. Pencharz, and R. O. Ball Dietary Cysteine Reduces the Methionine Requirement by an Equal Proportion in Both Parenterally and Enterally Fed Piglets J. Nutr., December 1, 2003; 133(12): 4215 - 4224. [Abstract] [Full Text] [PDF]
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J. W Miller, R. Green, M. I Ramos, L. H Allen, D. M Mungas, W. J Jagust, and M. N Haan Homocysteine and cognitive function in the Sacramento Area Latino Study on Aging Am. J. Clinical Nutrition, September 1, 2003; 78(3): 441 - 447. [Abstract] [Full Text] [PDF]
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A. K. Campbell, J. W. Miller, R. Green, M. N. Haan, and L. H. Allen Plasma Vitamin B-12 Concentrations in an Elderly Latino Population Are Predicted by Serum Gastrin Concentrations and Crystalline Vitamin B-12 Intake J. Nutr., September 1, 2003; 133(9): 2770 - 2776. [Abstract] [Full Text] [PDF]
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 R. A. Whitmer, M. N. Haan, J. W. Miller, and K. Yaffe Hormone Replacement Therapy and Cognitive Performance: The Role of Homocysteine J. Gerontol. A Biol. Sci. Med. Sci., April 1, 2003; 58(4): M324 - 330. [Abstract] [Full Text] [PDF]
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L. M Rogers, E. Boy, J. W Miller, R. Green, J. C. Sabel, and L. H Allen High prevalence of cobalamin deficiency in Guatemalan schoolchildren: associations with low plasma holotranscobalamin II and elevated serum methylmalonic acid and plasma homocysteine concentrations Am. J. Clinical Nutrition, February 1, 2003; 77(2): 433 - 440. [Abstract] [Full Text] [PDF]
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 J. W. Miller, M. I. Ramos, M. G. Garrod, M. A. Flynn, and R. Green Transcobalamin II 775G>C polymorphism and indices of vitamin B12 status in healthy older adults Blood, June 28, 2002; 100(2): 718 - 720. [Abstract] [Full Text] [PDF]
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J. W. Miller, R. Green, D. M. Mungas, B. R. Reed, and W. J. Jagust Homocysteine, vitamin B6, and vascular disease in AD patients Neurology, May 28, 2002; 58(10): 1471 - 1475. [Abstract] [Full Text] [PDF]
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J. Krijt, M. Vackova, and V. Kozich Measurement of Homocysteine and Other Aminothiols in Plasma: Advantages of Using Tris(2-carboxyethyl)phosphine as Reductant Compared with Tri-n-butylphosphine Clin. Chem., October 1, 2001; 47(10): 1821 - 1828. [Abstract] [Full Text] [PDF]
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C. M. Pfeiffer, S. P. Caudill, E. W. Gunter, B. A. Bowman, P. F. Jacques, J. Selhub, C. L. Johnson, D. T. Miller, and E. J. Sampson Analysis of Factors Influencing the Comparison of Homocysteine Values between the Third National Health and Nutrition Examination Survey (NHANES) and NHANES 1999+ J. Nutr., November 1, 2000; 130(11): 2850 - 2854. [Abstract] [Full Text] [PDF]
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M. Martinez, G. J. Cuskelly, J. Williamson, J. P. Toth, and J. F. Gregory III Vitamin B-6 Deficiency in Rats Reduces Hepatic Serine Hydroxymethyltransferase and Cystathionine {beta}-Synthase Activities and Rates of In Vivo Protein Turnover, Homocysteine Remethylation and Transsulfuration J. Nutr., May 1, 2000; 130(5): 1115 - 1123. [Abstract] [Full Text]
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C. M. Pfeiffer, D. L. Huff, S. J. Smith, D. T. Miller, and E. W. Gunter Comparison of Plasma Total Homocysteine Measurements in 14 Laboratories: An International Study Clin. Chem., August 1, 1999; 45(8): 1261 - 1268. [Abstract] [Full Text] [PDF]
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E. Causse, N. Siri, H. Bellet, S. Champagne, C. Bayle, P. Valdiguie, R. Salvayre, and F. Couderc Plasma Homocysteine Determined by Capillary Electrophoresis with Laser-induced Fluorescence Detection Clin. Chem., March 1, 1999; 45(3): 412 - 414. [Full Text] [PDF]
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C. M. Pfeiffer, D. L. Huff, and E. W. Gunter Rapid and Accurate HPLC Assay for Plasma Total Homocysteine and Cysteine in a Clinical Laboratory Setting Clin. Chem., February 1, 1999; 45(2): 290 - 292. [Full Text] [PDF]
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C. M. Pfeiffer, D. Twite, J. Shih, S. R. Holets-McCormack, and E. W. Gunter Method Comparison for Total Plasma Homocysteine between the Abbott IMx Analyzer and an HPLC Assay with Internal Standardization Clin. Chem., January 1, 1999; 45(1): 152 - 153. [Full Text] [PDF]
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V. C. Dias, F. J. Bamforth, M. Tesanovic, M. E. Hyndman, H. G. Parsons, and G. S. Cembrowski Evaluation and Intermethod Comparison of the Bio-Rad High-Performance Liquid Chromatographic Method for Plasma Total Homocysteine Clin. Chem., October 1, 1998; 44(10): 2199 - 2201. [Full Text] [PDF]
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D. W. Jacobsen Homocysteine and vitamins in cardiovascular disease Clin. Chem., August 1, 1998; 44(8): 1833 - 1843. [Abstract] [Full Text] [PDF]
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D. E. C. Cole, D. C. Lehotay, and J. Evrovski Simplified Simultaneous Assay of Total Plasma Homocysteine and Methionine by HPLC and Pulsed Integrated Amperometry Clin. Chem., January 1, 1998; 44(1): 188 - 190. [Full Text] [PDF]
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