Clinical Chemistry Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 43: 852-854, 1997;
This Article
Right arrow Full Text
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via ISI Web of Science (13)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lamont, J.
Right arrow Articles by FitzGerald, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lamont, J.
Right arrow Articles by FitzGerald, P.
Related Collections
Right arrow Nutrition
Right arrow Endocrinology and Metabolism
(Clinical Chemistry. 1997;43:852-854.)
© 1997 American Association for Clinical Chemistry, Inc.

Letters

Measurement of Individual vs Total Antioxidants

John Lamonta, John Campbell and Peter FitzGerald

Randox Laboratories Ltd., Ardmore, Diamond Rd., Crumlin BT29 4QY N. Ireland, UK
a Author for correspondence.


To the Editor:

Schofield and Braganza recently questioned [1] the accuracy and suitability of a Total Antioxidant Status (TAS) kit (Randox Labs., Crumlin, UK) for measuring total antioxidant capacity. They described data produced under reaction conditions not recommended by the manufacturer and reached incorrect conclusions based on the data obtained.

The changes in calculated TAS with reading time demonstrated by Schofield and Braganza (1) are supported by our own studies and are the reason why a 3-min time to reading is recommended in all analyzer applications. Observations made at alternative reading times are therefore not relevant when a standardized 3-min reading time is adhered to. Dilution of samples, which results in an increase in TAS, although not ideal, in practice is not necessary, given that we have never obtained a serum or plasma TAS value >2.5 mmol/L (linear limit of method). The lower recovery obtained by the authors is not in keeping with our own recovery studies, which have consistently achieved recoveries of >95%.

In all diagnostic tests, precise assay conditions must be selected and adhered to; with the availability of automated analyzers, these conditions can be easily achieved. The Randox TAS kit, therefore, allows many laboratories to perform TAS analyses under the same analytical conditions, leading to valid comparison of results between research groups.

Our data support the findings of Schofield and Braganza of different reaction kinetics for ascorbate, uric acid, and albumin. We believe, however, that the authors' key requirement that all antioxidants contribute to the total antioxidant capacity in an analogous manner is neither realistic nor necessary; antioxidants do not have the same reaction kinetics in vivo and may also interact with one another. It may be more meaningful, therefore, to measure the total antioxidants, given that the antioxidant . . . [Full Text of this Article]


References

David Schofielda and Joan M. Braganza

Pancreato-Biliary Service, Manchester Royal Infirmary, Oxford Rd., Manchester M13 9WL, UK
a Author for correspondence.


To the Editor:


References

John Lamont, John Campbell and Peter FitzGerald

,
a Author for correspondence.


To the Editor:


References






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1997 by the American Association for Clinical Chemistry.