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Clinical Chemistry 43: 1461-1462, 1997;
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(Clinical Chemistry. 1997;43:1461-1462.)
© 1997 American Association for Clinical Chemistry, Inc.

Technical Briefs

Prostate-Specific Antigen in Ascitic Fluid

Ferdinando Mannello1,a, Giovanni Miragoli2, Giuseppe Bianchi3 and Giancarlo Gazzanelli1

1 Ist. Istol. & Anal. Lab., Facoltà Sci. MFN Università, Via E. Zeppi, 61029 Urbino, Italia;
2 Div. Med. and
3 Lab. Anal. Ospedale Civile, Urbino, Italia;
a author for correspondence: fax +39-722-322370, e-mail mannello@bio.uniurb.it

Although previously thought to be produced almost exclusively by the epithelial cells of the prostate (1), prostate-specific antigen (PSA) is produced and secreted by several extraprostatic sources (2)(3). Considering the high degree of homology of PSA with the human glandular kallikrein (hKGK1) (4)(5) and the activation of the kallikrein–kinin system in peritoneal effusions (6)(7), we undertook the study of PSA distribution and expression in ascitic fluids. Between May 1996 and January 1997 we collected ascitic fluids from 44 consecutive women of ages 29–61 years (mean 49 ± 7) undergoing ultrasound examination and a diagnostic paracentesis. After collection (~10 mL), ascitic fluids were centrifuged at 20 120g for 20 min at +4 °C and the supernatants stored at -30 °C until processed. Blood samples were also taken, and after clotting were centrifuged at 360g for 5 min at +4 °C and stored at -30 °C until assay. In 24 patients (ages 28–81 years), the ascites was associated with malignancies (ovarian, pancreatic, breast, gastrointestinal, and lung). In the other 20 patients (18–75 years), ascites arose from chronic liver diseases, bacterial peritonitis, . . . [Full Text of this Article]


References




The following articles in journals at HighWire Press have cited this article:


Home page
 Clin. Chem.Home page
F. Mannello, M. Malatesta, F. Luchetti, S. Papa, S. Battistelli, and G. Gazzanelli
Immunoreactivity, Ultrastructural Localization, and Transcript Expression of Prostate-specific Antigen in Human Neuroblastoma Cell Lines
Clin. Chem., January 1, 1999; 45(1): 78 - 84.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
F. Mannello, F. Luchetti, D. Lancioli, S. Battistelli, S. Papa, and G. Gazzanelli
Prostate-specific Antigen Expression in Neoplastic Human Myeloid Cell Lines
Clin. Chem., September 1, 1998; 44(9): 1991 - 1993.
[Full Text] [PDF]


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