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Clinical Chemistry 43: 1798-1799, 1997;
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(Clinical Chemistry. 1997;43:1798-1799.)
© 1997 American Association for Clinical Chemistry, Inc.

Oak Ridge Poster Sessions

Clinical Application of Capillary Isoelectric Focusing on Fused Silica Capillary for Determination of Hemoglobin Variants

Amin A. Mohammada, Anthony O. Okorodudu, Michael G. Bissell, Pat Dow, Gregg Reger1, Annette Meier1, Phil Guodagno1 and John R. Petersen

1 Helena Labs., Beaumont, TX;
a author for correspondence: fax 409-772-9231

Hemoglobin is composed of two {alpha} and two ß polypeptide chains. Any modifications in the amino acid sequence, which may either be congenital or acquired, affects the oxygen-carrying ability of hemoglobin, resulting in a series of hemoglobinopathies. To date, >600 structurally different human hemoglobins are known (1)(2). Identification of abnormal hemoglobin is very important in the differential diagnosis of hemoglobinopathies. Currently, most clinical laboratories have a battery of hemoglobin assays that includes gel electrophoresis, ion-exchange or affinity chromatography, and isoelectric focusing (1)(3). Of all the available methods, computer-operated cation-exchange HPLC is the most widely used for identifying and quantifying major and minor hemoglobins (4)(5)(6)(7). The main disadvantage of a HPLC system is expensive instrumentation and the high cost of columns and reagents. Although an excellent resolution is achieved with gel isoelectric focusing (8), it is labor intensive, time consuming, and not readily amenable for routine screening.

The use of capillary electrophoresis (CE) for identification of hemoglobin variants has been reported by several authors (9)(10)(11)(12. . . [Full Text of this Article]


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Copyright © 1997 by the American Association for Clinical Chemistry.