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Oak Ridge Poster Sessions |
1
Becton Dickinson Immunocytometry Systems, 2350 Qume Dr., San Jose, CA 95131-1807, and
2
Pharmingen, San Diego, CA 92121;
a author for correspondence: fax 408-954-2156, e-mail roy_chen@bdis.com
The study of cytokines has been of great interest to researchers in many scientific disciplines, including cell biology and immunology. Cytokines form a sophisticated network that serves to modulate a myriad of cellular events. Within such a network, and through complex feedback mechanisms, cytokine functions are mostly interdependent. Because of the extreme complexity of the cytokine network, a simultaneous measurement of multiple cytokines in a single sample represents a desirable and effective approach.
Several methods are available to measure cytokines and their messenger
RNAs. To measure cytokines secreted from cells, researchers commonly
use conventional ELISA techniques. ELISA methods are generally
cost-effective but restricted to measuring one cytokine at a time.
Consequently, the ELISA approach requires not only multiple sample
aliquots but also repetitive execution of the procedures for each
cytokine of interest. The ability of flow cytometry to simultaneously
acquire data for numerous particles and subsequently analyze multiple
characteristics for each particle makes it a powerful technique
for cell sorting and cell analyses. The application is fundamentally
built on the high sensitivity of a flow cytometer to discern
characteristics either within or on the surface of a cell. Several
investigators have constructed microparticle-based immunoassays that
use a flow cytometer
Indirect format.
Direct format.
References
The following articles in journals at HighWire Press have cited this article:
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  H. R. Wong, N. Cvijanovich, D. S. Wheeler, M. T. Bigham, M. Monaco, K. Odoms, W. L. Macias, and M. D. Williams Interleukin-8 as a Stratification Tool for Interventional Trials Involving Pediatric Septic Shock Am. J. Respir. Crit. Care Med., August 1, 2008; 178(3): 276 - 282. [Abstract] [Full Text] [PDF]
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 K. G.-J. Ooi, G. Galatowicz, H. M. A. Towler, S. L. Lightman, and V. L. Calder Multiplex Cytokine Detection versus ELISA for Aqueous Humor: IL-5, IL-10, and IFN{gamma} Profiles in Uveitis Invest. Ophthalmol. Vis. Sci., January 1, 2006; 47(1): 272 - 277. [Abstract] [Full Text] [PDF]
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 D. Liu, C. Li, Y. Chen, C. Burnett, X. Y. Liu, S. Downs, R. D. Collins, and J. Hawiger Nuclear Import of Proinflammatory Transcription Factors Is Required for Massive Liver Apoptosis Induced by Bacterial Lipopolysaccharide J. Biol. Chem., November 12, 2004; 279(46): 48434 - 48442. [Abstract] [Full Text] [PDF]
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 S. Faucher, A. Martel, A. Sherring, T. Ding, L. Malloch, J. E. Kim, M. Bergeron, P. Sandstrom, and F. F. Mandy Protein Bead Array for the Detection of HIV-1 Antibodies from Fresh Plasma and Dried-Blood-Spot Specimens Clin. Chem., July 1, 2004; 50(7): 1250 - 1253. [Full Text] [PDF]
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D. Liu, X. Y. Liu, D. Robinson, C. Burnett, C. Jackson, L. Seele, R. A. Veach, S. Downs, R. D. Collins, D. W. Ballard, et al. Suppression of Staphylococcal Enterotoxin B-induced Toxicity by a Nuclear Import Inhibitor J. Biol. Chem., April 30, 2004; 279(18): 19239 - 19246. [Abstract] [Full Text] [PDF]
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A. Tarnok, J. Hambsch, R. Chen, and R. Varro Cytometric Bead Array to Measure Six Cytokines in Twenty-Five Microliters of Serum Clin. Chem., June 1, 2003; 49(6): 1000 - 1002. [Full Text] [PDF]
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 J. F. McDyer, Z. Li, S. John, X. Yu, C.-y. Wu, and J. A. Ragheb IL-2 Receptor Blockade Inhibits Late, But Not Early, IFN-{gamma} and CD40 Ligand Expression in Human T Cells: Disruption of Both IL-12-Dependent and -Independent Pathways of IFN-{gamma} Production J. Immunol., September 1, 2002; 169(5): 2736 - 2746. [Abstract] [Full Text] [PDF]
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L. S. Metelitsa, O. V. Naidenko, A. Kant, H.-W. Wu, M. J. Loza, B. Perussia, M. Kronenberg, and R. C. Seeger Human NKT Cells Mediate Antitumor Cytotoxicity Directly by Recognizing Target Cell CD1d with Bound Ligand or Indirectly by Producing IL-2 to Activate NK Cells J. Immunol., September 15, 2001; 167(6): 3114 - 3122. [Abstract] [Full Text] [PDF]
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