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Clinical Chemistry 46: 1717-1719, 2000;
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(Clinical Chemistry. 2000;46:1717-1719.)
© 2000 American Association for Clinical Chemistry, Inc.

Letters

Evaluation of a Simple Dot-Blot Method for the Detection of Anti-Neutrophil Cytoplasmic Antibodies Directed against Proteinase 3 and Myeloperoxidase

Anthony A.M. Ermens1,a, Angelique J.M. Bayens1, Adriënne Crooymans1, Anita A.M. Broekman-van Hout2 and Hans L.P. van Duijnhoven2

1 Klinisch Laboratorium, Diaconessenhuis, Postbus 90052, 5600 PD Eindhoven, The Netherlands

2 Algemeen Klinisch Laboratorium, Elkerliek Ziekenhuis, 5800 AB Helmond, The Netherlands
a Author for correspondence. Fax 31-40-2335595; e-mail termens@iae.nl.


To the Editor:

Anti-neutrophil cytoplasmic antibodies (ANCAs) usually are demonstrated by indirect immunofluorescence (IF), followed by examination of patient samples for the antibodies against proteinase-3 (PR3) and myeloperoxidase (MPO). The antibody testing usually requires ELISA and may be hampered by the cost and the requirement for an ELISA reader. Dot-blot (DB) analysis can be an attractive alternative for ELISA for the identification of some autoantibodies (1)(2).

In this study, we compared a capture ELISA for PR3 and MPO antibodies with a new, commercially available DB method. Sera from 74 patients were selected for the evaluation. In addition, samples with negative IF results (n = 40); samples with cytoplasmic (c)-ANCAs (n = 20), perinuclear (p)-ANCAs (n = 8), or atypical IF (n = 4) results; and samples with equivocal IF results (n = 4) were included.

Routine detection of antibodies . . . [Full Text of this Article]


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