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Clinical Chemistry 46: 1989-1990, 2000;
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(Clinical Chemistry. 2000;46:1989-1990.)
© 2000 American Association for Clinical Chemistry, Inc.

Technical Briefs

Analytical and Clinical Performance of the Immulite Cardiac Troponin I Assay

Alain Lavoinne1,a, Bruno Cauliez1, Hélène Eltchaninoff2, René Koning2 and Alain Cribier2

1 Laboratoire de Biochimie Médicale and
2 Service de Cardiologie, Hôpital Charles Nicolle, CHUR de Rouen, 76031 Rouen cedex, France
a author for correspondence: fax 33-2-32-88-87-80, e-mail alain.lavoinne@chu-rouen.fr

Cardiac troponin I (cTnI) is now regarded as one of the most specific markers for cardiac injury, and an increasing number of commercial methods are available. We report here a study of the Immulite cTnI assay manufactured by DPC.

The Immulite cTnI assay is a sandwich immunoassay that uses a monoclonal antibody immobilized on beads and a goat polyclonal antibody labeled with alkaline phosphatase as a tracer (1); both antibodies recognize epitopes localized in the N-terminal part (residues 33–110) of the protein. The chemiluminescent substrate used for the enzymatic reaction is an ester of adamantyl dioxetane phosphate. We used as calibrators purified human cTnI in horse serum. The within- and between-run imprecisions (CV) were <3.9% and 3.6%, respectively, at a mean cTnI of 1.5 µg/L (n = 20). The minimal cTnI value quantified was 0.2 µg/L, and no detectable cTnI was observed in 20 runs of assay diluent. The lowest . . . [Full Text of this Article]


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The following articles in journals at HighWire Press have cited this article:


Home page
 Clin. Chem.Home page
A. Lavoinne, B. Cauliez, H. Eltchaninoff, C. Tron, and A. Cribier
Release of Macromolecular Cardiac Troponin I Complex after Successful Percutaneous Transluminal Coronary Angioplasty in Acute Myocardial Infarction
Clin. Chem., March 1, 2003; 49(3): 505 - 507.
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Home page
 Clin. Chem.Home page
V. Scharnhorst, H. L. Vader, and F. van der Graaf
Characteristics of the Cardiac Troponin I Assay on the Immulite 2000 Analyzer
Clin. Chem., September 1, 2002; 48(9): 1626 - 1627.
[Full Text] [PDF]


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