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Clinical Chemistry 46: 736-737, 2000;
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(Clinical Chemistry. 2000;46:736-737.)
© 2000 American Association for Clinical Chemistry, Inc.

Letters

Interference of Iodinated Contrast Media in Serum Capillary Zone Electrophoresis

María Luisa Arranz-Peñaa,1, Manuel González-Sagrado1, Ana María Olmos-Linares1, Nuria Fernández-García1 and Francisco Javier Martín-Gil1

1 Laboratory of Clinical Biochemistry, University Hospital "Del Río Hortega", Cardenal Torquemada, s/n, 47010 Valladolid, Spain
a Author for correspondence. Fax 34-983-331566.


To the Editor:

Automated capillary zone electrophoresis (e.g., on the Paragon 2000 CZE, Beckman Instruments) is an alternative to conventional electrophoretic methods for the separation of serum proteins (1)(2)(3). The technique uses ultraviolet (UV) detection at 214 nm for direct quantification of peptide bonds, whereas conventional methods detect the protein fractions by staining with various reagents.

CZE has the advantage of improved precision and a faster turnaround (4). Recent reports indicate, however, that the Paragon CZE 2000 masks deficiencies of {alpha}1-antitrypsin (5) and that some radio-opaque agents simulate a monoclonal component in the {alpha}2-globulin fraction in the CZE electropherogram (6)(7).

We encountered an interference from the contrast medium Omnitrast®, which produced an unexpected peak in the {alpha}2-zone of the CZE electropherogram. We have now investigated the effect of 12 iodinated radio-opaque media (commercially available in Spain) on instrumentation (Paragon CZE 2000) operated as described by Bossuyt et al. (6). When we used . . . [Full Text of this Article]


Acknowledgments


References




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