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Agreement Study of Methods Based on the Elimination Principle for the Measurement of LDL- and HDL-Cholesterol Compared with Ultracentrifugation in Patients with Liver Cirrhosis

¹ Centre de Recerca Biomèdica, Hospital Universitari de Sant Joan, 43201-Reus, Catalunya, Spain
^a address correspondence to this author at: C/. Sant Joan s/n, 43201-Reus, Catalunya, Spain: fax 34-977-312569, e-mail jcamps@grupsgs.com

In recent years, several methods for the direct measurement of HDL-cholesterol (HDL-C) and LDL-cholesterol (LDL-C) have been introduced (1)(2)(3)(4)(5). These methods have been effective and inexpensive tools for the routine screening of large populations because they prevent the need for complex ultracentrifugation procedures. In addition, the direct methods for LDL-C are better than indirect calculation by the Friedewald formula because they can be used accurately in hypertriglyceridemic patients and in nonfasting subjects (4). However, it is still being debated whether these methods are also valuable under more challenging conditions than those found in the general population (4), i.e., pathological circumstances in which there are abnormalities in lipoprotein structure and/or composition.

In a recent study, we observed that three different homogeneous methods significantly undervalue HDL-C concentrations in patients with liver cirrhosis. We suggested that this could be related to the abnormal lipoprotein composition in these subjects (6)(7). A new type of homogeneous method, based on the elimination principle (Randox Laboratories Ltd., Crumlin, UK), has been developed for measuring HDL-C and LDL-C. The manufacturer states that lipoproteins with abnormal compositions may be correctly identified and measured with these methods. The present study therefore aimed to compare the elimination methods for determining homogeneous HDL-C and LDL-C with single vertical-spin ultracentrifugation in a group of patients with liver cirrhosis.

The study was carried out on 44 control subjects and 55 cirrhotic patients. Control subjects were chosen randomly from the routine health and safety-at-work checks conducted in several companies in our area. Cirrhotic patients were diagnosed by liver biopsy and came from the outpatient . . . [Full Text of this Article]

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References

The following articles in journals at HighWire Press have cited this article:

				W. G. Miller, P. P. Waymack, F. P. Anderson, S. F. Ethridge, and E. C. Jayne Performance of Four Homogeneous Direct Methods for LDL-Cholesterol Clin. Chem., March 1, 2002; 48(3): 489 - 498. [Abstract] [Full Text] [PDF]

				G. R. Warnick, M. Nauck, and N. Rifai Evolution of Methods for Measurement of HDL-Cholesterol: From Ultracentrifugation to Homogeneous Assays Clin. Chem., September 1, 2001; 47(9): 1579 - 1596. [Abstract] [Full Text] [PDF]